Treatment of Chlorella sp. extract on heat shock cluster (HSC) response from the tissue and bloodcells proliferation of Epinephelus fuscoguttatus-lanceolatus infected by Viral Nervous Necrosis

VNN infection can trigger an immune response, inflammation in fish cells and tissuessuch as Heat Shock Cluster (HSC).The objective of this study was to determine the presence of HSC responses in Cantang groupers (E. fuscoguttatus-lanceolatus) that infected by VNN and treated with Chlorella sp extract.The methods of the study included the preparation of Chlorella sp extract, infecting fish with VNN, testing the comparison among the treatments.The treatment of C.vulgaris was(K): grouper without treatment with both C.vulgaris and VNN), (V):treatment of VNN without C.vulgaris, (A1): treatment of C.vulgaris 17 g/ml, (A2): treatment of C.vulgaris33 g/ml, (A3) treatment of C.vulgaris 50 g/ml, (AV1):treatment of C.vulgaris 17 g/ml and VNN, (AV2): treatment of C.vulgari 33 g/ml and VNN, (AV3): treatment C.vulgaris 50 g/ml and VNN. The results analysis included blood cell and tissue observation using immunohistochemistry and immunoratio, and confirmation of HSC assay results with inhibition test of grouper cell blood labeled with anti-mouse antibody IgG, and secondary antibody IgG anti-mouse SA-HRP. The results showed that Chlorella sp extract administered to VNN-infected grouper and fish without VNN infection, significantly increase the HSC response.