Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae)

Sayyidatul Awalia Nuzula; Zulfaidah Penata Gama

doi:10.1063/1.5061905

Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae)

Sayyidatul Awalia Nuzula^*
, Zulfaidah Penata Gama

^*Corresponding author for this work

Faculty of Mathematics and Natural Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Dengue hemorrhagic fever is caused by dengue viruses that infect female mosquitoes, mainly of the species Aedes aegypti and to a lesser extent Aedes albopictus. One example of a bioinsecticide is Bacillus thuringiensis israelensis (Bti), which is a naturally occurring soil bacterium that can effectively kill mosquito larvae present in water. MOSNON^TM is a biolarvicide product containing B. thuringiensis. The aims of this research were to analyze the effects of MOSNON^TM toward mortality and behavior of the non-target organism Daphnia sp. This study was conducted at the Laboratory of Ecology and Animal Diversity and Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Brawijaya University. The toxicity test used 10 neonates of Daphnia sp. for each bottle that contained 250 mL MOSNON^TM solution. Toxicity test was divided into several concentrations of MOSNON^TM, they were 0, 5, 10, 15 and 20 ppm, and each experiment was repeated four times. Observations of Daphnia sp. mortality and behavior as well as abiotic factors (temperature, dissolved oxygen and pH) were conducted at exposure times of 0, 24, 48, 72 and 96 hours. Data were analyzed using ANOVA. The results showed that all concentrations of MOSNON had no effect on mortality or behavior of Daphnia sp. MOSNON^TM is a potential biolarvicide for mosquitoes and safe for non-target organisms (Daphnia sp.).

Original language	English
Title of host publication	9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University
Editors	Fahrul Zaman Bin Huyop, Dian Siswanto, Retno Mastuti, Chairat Treesubsuntorn
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735417373
DOIs	https://doi.org/10.1063/1.5061905
Publication status	Published - 10 Oct 2018
Event	9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018 - Malang City - East Java, Indonesia Duration: 7 Mar 2018 → 8 Mar 2018

Publication series

Name	AIP Conference Proceedings
Volume	2019
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018
Country/Territory	Indonesia
City	Malang City - East Java
Period	7/03/18 → 8/03/18

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bioinsecticide
dengue hemorrhagic fever
MOSNON™

Access to Document

10.1063/1.5061905

Cite this

Nuzula, S. A., & Gama, Z. P. (2018). Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae). In F. Z. B. Huyop, D. Siswanto, R. Mastuti, & C. Treesubsuntorn (Eds.), 9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University Article 050012 (AIP Conference Proceedings; Vol. 2019). American Institute of Physics Inc.. https://doi.org/10.1063/1.5061905

Nuzula, Sayyidatul Awalia ; Gama, Zulfaidah Penata. / Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae). 9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University. editor / Fahrul Zaman Bin Huyop ; Dian Siswanto ; Retno Mastuti ; Chairat Treesubsuntorn. American Institute of Physics Inc., 2018. (AIP Conference Proceedings).

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title = "Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae)",

abstract = "Dengue hemorrhagic fever is caused by dengue viruses that infect female mosquitoes, mainly of the species Aedes aegypti and to a lesser extent Aedes albopictus. One example of a bioinsecticide is Bacillus thuringiensis israelensis (Bti), which is a naturally occurring soil bacterium that can effectively kill mosquito larvae present in water. MOSNONTM is a biolarvicide product containing B. thuringiensis. The aims of this research were to analyze the effects of MOSNONTM toward mortality and behavior of the non-target organism Daphnia sp. This study was conducted at the Laboratory of Ecology and Animal Diversity and Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Brawijaya University. The toxicity test used 10 neonates of Daphnia sp. for each bottle that contained 250 mL MOSNONTM solution. Toxicity test was divided into several concentrations of MOSNONTM, they were 0, 5, 10, 15 and 20 ppm, and each experiment was repeated four times. Observations of Daphnia sp. mortality and behavior as well as abiotic factors (temperature, dissolved oxygen and pH) were conducted at exposure times of 0, 24, 48, 72 and 96 hours. Data were analyzed using ANOVA. The results showed that all concentrations of MOSNON had no effect on mortality or behavior of Daphnia sp. MOSNONTM is a potential biolarvicide for mosquitoes and safe for non-target organisms (Daphnia sp.).",

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Nuzula, SA & Gama, ZP 2018, Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae). in FZB Huyop, D Siswanto, R Mastuti & C Treesubsuntorn (eds), 9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University., 050012, AIP Conference Proceedings, vol. 2019, American Institute of Physics Inc., 9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018, Malang City - East Java, Indonesia, 7/03/18. https://doi.org/10.1063/1.5061905

Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae). / Nuzula, Sayyidatul Awalia; Gama, Zulfaidah Penata.
9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University. ed. / Fahrul Zaman Bin Huyop; Dian Siswanto; Retno Mastuti; Chairat Treesubsuntorn. American Institute of Physics Inc., 2018. 050012 (AIP Conference Proceedings; Vol. 2019).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae)

AU - Nuzula, Sayyidatul Awalia

AU - Gama, Zulfaidah Penata

PY - 2018/10/10

Y1 - 2018/10/10

N2 - Dengue hemorrhagic fever is caused by dengue viruses that infect female mosquitoes, mainly of the species Aedes aegypti and to a lesser extent Aedes albopictus. One example of a bioinsecticide is Bacillus thuringiensis israelensis (Bti), which is a naturally occurring soil bacterium that can effectively kill mosquito larvae present in water. MOSNONTM is a biolarvicide product containing B. thuringiensis. The aims of this research were to analyze the effects of MOSNONTM toward mortality and behavior of the non-target organism Daphnia sp. This study was conducted at the Laboratory of Ecology and Animal Diversity and Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Brawijaya University. The toxicity test used 10 neonates of Daphnia sp. for each bottle that contained 250 mL MOSNONTM solution. Toxicity test was divided into several concentrations of MOSNONTM, they were 0, 5, 10, 15 and 20 ppm, and each experiment was repeated four times. Observations of Daphnia sp. mortality and behavior as well as abiotic factors (temperature, dissolved oxygen and pH) were conducted at exposure times of 0, 24, 48, 72 and 96 hours. Data were analyzed using ANOVA. The results showed that all concentrations of MOSNON had no effect on mortality or behavior of Daphnia sp. MOSNONTM is a potential biolarvicide for mosquitoes and safe for non-target organisms (Daphnia sp.).

AB - Dengue hemorrhagic fever is caused by dengue viruses that infect female mosquitoes, mainly of the species Aedes aegypti and to a lesser extent Aedes albopictus. One example of a bioinsecticide is Bacillus thuringiensis israelensis (Bti), which is a naturally occurring soil bacterium that can effectively kill mosquito larvae present in water. MOSNONTM is a biolarvicide product containing B. thuringiensis. The aims of this research were to analyze the effects of MOSNONTM toward mortality and behavior of the non-target organism Daphnia sp. This study was conducted at the Laboratory of Ecology and Animal Diversity and Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Brawijaya University. The toxicity test used 10 neonates of Daphnia sp. for each bottle that contained 250 mL MOSNONTM solution. Toxicity test was divided into several concentrations of MOSNONTM, they were 0, 5, 10, 15 and 20 ppm, and each experiment was repeated four times. Observations of Daphnia sp. mortality and behavior as well as abiotic factors (temperature, dissolved oxygen and pH) were conducted at exposure times of 0, 24, 48, 72 and 96 hours. Data were analyzed using ANOVA. The results showed that all concentrations of MOSNON had no effect on mortality or behavior of Daphnia sp. MOSNONTM is a potential biolarvicide for mosquitoes and safe for non-target organisms (Daphnia sp.).

KW - Bioinsecticide

KW - dengue hemorrhagic fever

KW - MOSNON™

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U2 - 10.1063/1.5061905

DO - 10.1063/1.5061905

M3 - Conference contribution

AN - SCOPUS:85055525059

T3 - AIP Conference Proceedings

BT - 9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University

A2 - Huyop, Fahrul Zaman Bin

A2 - Siswanto, Dian

A2 - Mastuti, Retno

A2 - Treesubsuntorn, Chairat

PB - American Institute of Physics Inc.

T2 - 9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018

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ER -

Nuzula SA, Gama ZP. Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae). In Huyop FZB, Siswanto D, Mastuti R, Treesubsuntorn C, editors, 9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University. American Institute of Physics Inc. 2018. 050012. (AIP Conference Proceedings). doi: 10.1063/1.5061905

Toxicity studies of Bacillus thuringiensis on non-target organism Daphnia sp. (Diplostraca: Daphniidae)

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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