Topical ozonated virgin coconut oil improves diabetic ulcer wound healing in diabetic mice model

Diabetic ulcers are open sores on the skin through into the dermis, which if not properly managed, can increase amputation cases. Over the past few decades, ozone generated using plasma medical technology has been investigated to have the ability as an agent that helps wound healing. This study aims to evaluate the effect of topical ozonated VCO on the diabetic wound healing in the diabetic mice model. This study was an experimental study with post-test control design. Ulcer wound model was made in 50 diabetic male Wistar mice. They are divided into 5 groups, the first group (control) was given conventional therapy and the other groups (treatment) were given conventional therapy and topical ozonated VCO with different flow durations (0 min, 90 min, 7 h, 14 h). Then, the characteristics of wound healing (macroscopic and wound lengths) were observed in day 1, 3, 5, 7, and 14. The results of this study showed that the reduction of wound length was proportionally related to the duration of ozone flow. Topical VCO with the longer duration of ozone flow would heal the wound more quickly and had the shortest wound length at the end of the observation. VCO with ozone flow for 14 hours (16837.10 µm) had the biggest reduction of wound length, following by VCO with ozone flow for 7 hours (14209.64 µm), 90 minutes (14071.96 µm), 0 minutes (8531.99 µm), and control group (6370.77 µm). Therefore, we concluded that topical ozonated VCO improved diabetic wound healing process in diabetic ulcer mice model and can be used as adjuvant therapy for diabetic ulcers.