Thyroid stimulating hormone (TSH) and thyroxine hormone (T4) role in thyroid detriment on hypothyroidsm animal models

Hypothyroidism is a condition where thyroid gland encounter on degradation. It causes many complication impacts especially on hormone metabolism. This previous study had developed the preparation of hypothyroidism animal model by injected of goat Thyroglobulin (cTg) through detriment of tyroid gland, as well as other impact to rat (Rattus norvegicus) as experimental animal model. This study was followed by optimizing the preparation also the exhaustively observation of rat animal model. Based on previous worked, preparation of animal model were conducted by intravenous injected of 200 μg/mL of goat thyroglobulin (cTg) with Complete Freund’s Adjuvant (CFA) at 1:1 ratio continued with booster on Incomplete Freund’s Adjuvant (IFA) at same ratio. Then levelthyroid stimulating hormone (TSH)and thyroxine hormone (T4) were measured then analyzed with the alteration on thyroid gland to evaluate the development of the disease. This research showed, injection of cTg could lead to decrease of T4 levels and increase TSH levels. Meanwhile the thyroid gland which were had been injected with cTg showed the different size, structure also the mononuclear cells infiltration compare to control group. The binding of cTg and thyroid follicle caused damage and dysfunction of thyroid gland. These findings showed the hypothyroidism condition can alter other hormone produced gland which lead to development of diseases.