The role of VipAlbumin® as an immunostimulatory agent for controlling homeostasis and proliferation of lymphoid cells

VipAlbumin® is a supplement from snakehead fish (Ophiocephalus striatus) which has high content of albumin that is very important to develop new cells. The aims of this study were to know the effect of VipAlbumin® to cell proliferation, expression level of CD4⁺CD62L⁺ T cell, regulatory T cell, and B220+ cell, and immunocompetent cell cycle. Cell isolated from spleen of pathogen free mice were cultured in RPMI 1640 with 10% FBS, 1% Pen/Strep 10 ×, 2-Mercaptoetanol, anti-CD3 and LPS. The concentrations of VipAlbumin® used were 0 μg/ml; 0.33 μg/ml; 33.3 μg/ml; and 3333.3 μg/ml. The cell was incubated in CO₂ 5% incubator 37oC for 3 days for cell cycle and 5 days for proliferation analysis and cell expression. FACS analysis was done to know cell proliferation profile, status of cell, and cell cycle. Concentration 33.3 μg/ml and 3333.3 μg/ml significantly can increase cell proliferation and induce cell enter G2/M phase (p < 0.05) compared to control. VipAlbumin can significantly increase the relative number of CD4⁺CD62L⁺ T cell, regulatory T cell, and B220+ cell (p < 0.05) compared to control. This study gives scientific evidence that VipAlbumin can be used as an immunostimulant which accelerates immunocompetent cells growth.