The role of collagen scaffold and stromal vascular fraction on healing process in growth plate injury (SOX9 and histological examination)

BACKGROUND: Pediatric skeletal trauma presents a distinct approach to its treatment because of its unique auses and possibility of its troublesome complications. One of its major complications is growth plate injury which may disturb the longitudinal growth of child’s bone. AIM: In this study, the author combined stromal vascular fraction (SVF) and collagen scaffold as biomaterial for future treatment of physeal injury in skeletally immature patients using SOX9 and histological examination as a marker. METHODS: The study was conducted experimentally on Rattus norvegicus growth plate based on Erickson study, and the SVF was from R. norvegicus fat tissue with ZUK method. Histological examination was evaluated with 8 times magnification and SOX9 from the growth plate was measured with enzyme-linked immunosorbent assay. RESULTS: Histological examination showed that the best result was obtained in SVF + collagen group judging by the bony bridge diameter. The result was differed significantly from the positive control group with p < 0.05 in Tukey test. The result from SOX9 level measurement shows that all of the treatment groups SOX9 level almost the same as normal value (negative control group) and it is significantly differ from the positive control group with p < 0.05 in Mann–Whitney U-test. CONCLUSIONS: This study showed that the combination of collagen and SVF had a great effect on healing process in growth plate injury.