The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat

Elisa Danik Kurniawati; Tanti Tri Lestary; Ni Putu Sri Haryati; Eviana Norahmawati; I. Wayan Arsana Wiyasa; Tatit Nurseta; Dwi Yuni Hidayati; Loeki Enggar Fitri

doi:10.1063/5.0117826

The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat

Elisa Danik Kurniawati
, Tanti Tri Lestary
, Ni Putu Sri Haryati
, Eviana Norahmawati
, I. Wayan Arsana Wiyasa^*
, Tatit Nurseta
, Dwi Yuni Hidayati
, Loeki Enggar Fitri

^*Corresponding author for this work

Faculty of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Menopause can lead to health problems such as osteoporosis, neurological and cardiovascular disease. Neurologically, Alzheimer's is caused by the accumulation of amyloid peptides that form plaques in the brain resulting in neuronal death. In addition, the incidence of osteoporosis also increases during menopause which is indicated by an increase in bone RANKL expression. Nitric Oxide (NO) is a modulator of endothelial cell permeability, which shows an association with cardiovascular diseases. Genistein in cowpea is expected to have a protective effect. The study aims to prove that cowpea can reduce brain amyloid β, bone RANKL expression, NO levels of an ovariectomized rat. Twenty-four Rattus norvegicus females were divided into a control group (SHAM), an OVX group given 17-estradiol 0.18 mg/kg BW/day, and three OVX groups given extracts 1.25, 2.5, and 5 mg/kg. BB/day for 40 days. Brain amyloid β expression was measured by immunofluorescence, bone RANKL expression was observed by immunohistochemical staining, and NO levels by colorimetry method. Cowpea extract can be used as an antioxidant agent and was shown to significantly reduce the expression of brain amyloid β (p value 0.000), bone RANKL (p value 0.006), and no significant difference for NO levels (p value 0.162) in ovariectomized rats.

Original language	English
Title of host publication	4th International Conference on Life Science and Technology, ICoLiST 2023
Editors	Ahmad Taufiq, Hendra Susanto, I. Ching Wang, Hadi Nur, Muhammad Aziz, Chuang-Rung Chang, Gereon Elbers, Sri Rahayu Lestari, Markus Diantoro, Nandang Mufti, Chen Siang Ng, Nik Ahmad Nizam Nik Malek, Sunaryono, Yu-Hsin Chiu, Siti Zubaidah, Aulanni'am, Indra Wibowo, Adeodatus Yuda Handaya
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735443136
DOIs	https://doi.org/10.1063/5.0117826
Publication status	Published - 24 Jan 2023
Event	4th International Conference on Life Science and Technology, ICoLiST 2023 - Virtual, Online, Indonesia Duration: 31 Aug 2021 → …

Publication series

Name	AIP Conference Proceedings
Volume	2634
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	4th International Conference on Life Science and Technology, ICoLiST 2023
Country/Territory	Indonesia
City	Virtual, Online
Period	31/08/21 → …

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1063/5.0117826

Cite this

Kurniawati, E. D., Lestary, T. T., Haryati, N. P. S., Norahmawati, E., Wiyasa, I. W. A., Nurseta, T., Hidayati, D. Y., & Fitri, L. E. (2023). The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat. In A. Taufiq, H. Susanto, I. C. Wang, H. Nur, M. Aziz, C.-R. Chang, G. Elbers, S. R. Lestari, M. Diantoro, N. Mufti, C. S. Ng, N. A. N. N. Malek, Sunaryono, Y.-H. Chiu, S. Zubaidah, Aulanni'am, I. Wibowo, & A. Y. Handaya (Eds.), 4th International Conference on Life Science and Technology, ICoLiST 2023 Article 020032 (AIP Conference Proceedings; Vol. 2634). American Institute of Physics Inc.. https://doi.org/10.1063/5.0117826

Kurniawati, Elisa Danik ; Lestary, Tanti Tri ; Haryati, Ni Putu Sri et al. / The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat. 4th International Conference on Life Science and Technology, ICoLiST 2023. editor / Ahmad Taufiq ; Hendra Susanto ; I. Ching Wang ; Hadi Nur ; Muhammad Aziz ; Chuang-Rung Chang ; Gereon Elbers ; Sri Rahayu Lestari ; Markus Diantoro ; Nandang Mufti ; Chen Siang Ng ; Nik Ahmad Nizam Nik Malek ; Sunaryono ; Yu-Hsin Chiu ; Siti Zubaidah ; Aulanni'am ; Indra Wibowo ; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2023. (AIP Conference Proceedings).

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title = "The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat",

abstract = "Menopause can lead to health problems such as osteoporosis, neurological and cardiovascular disease. Neurologically, Alzheimer's is caused by the accumulation of amyloid peptides that form plaques in the brain resulting in neuronal death. In addition, the incidence of osteoporosis also increases during menopause which is indicated by an increase in bone RANKL expression. Nitric Oxide (NO) is a modulator of endothelial cell permeability, which shows an association with cardiovascular diseases. Genistein in cowpea is expected to have a protective effect. The study aims to prove that cowpea can reduce brain amyloid β, bone RANKL expression, NO levels of an ovariectomized rat. Twenty-four Rattus norvegicus females were divided into a control group (SHAM), an OVX group given 17-estradiol 0.18 mg/kg BW/day, and three OVX groups given extracts 1.25, 2.5, and 5 mg/kg. BB/day for 40 days. Brain amyloid β expression was measured by immunofluorescence, bone RANKL expression was observed by immunohistochemical staining, and NO levels by colorimetry method. Cowpea extract can be used as an antioxidant agent and was shown to significantly reduce the expression of brain amyloid β (p value 0.000), bone RANKL (p value 0.006), and no significant difference for NO levels (p value 0.162) in ovariectomized rats.",

author = "Kurniawati, \{Elisa Danik\} and Lestary, \{Tanti Tri\} and Haryati, \{Ni Putu Sri\} and Eviana Norahmawati and Wiyasa, \{I. Wayan Arsana\} and Tatit Nurseta and Hidayati, \{Dwi Yuni\} and Fitri, \{Loeki Enggar\}",

note = "Publisher Copyright: {\textcopyright} 2023 American Institute of Physics Inc.. All rights reserved.; 4th International Conference on Life Science and Technology, ICoLiST 2023 ; Conference date: 31-08-2021",

year = "2023",

month = jan,

day = "24",

doi = "10.1063/5.0117826",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Ahmad Taufiq and Hendra Susanto and Wang, \{I. Ching\} and Hadi Nur and Muhammad Aziz and Chuang-Rung Chang and Gereon Elbers and Lestari, \{Sri Rahayu\} and Markus Diantoro and Nandang Mufti and Ng, \{Chen Siang\} and Malek, \{Nik Ahmad Nizam Nik\} and Sunaryono and Yu-Hsin Chiu and Siti Zubaidah and Aulanni'am and Indra Wibowo and Handaya, \{Adeodatus Yuda\}",

booktitle = "4th International Conference on Life Science and Technology, ICoLiST 2023",

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Kurniawati, ED, Lestary, TT, Haryati, NPS, Norahmawati, E, Wiyasa, IWA, Nurseta, T, Hidayati, DY & Fitri, LE 2023, The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat. in A Taufiq, H Susanto, IC Wang, H Nur, M Aziz, C-R Chang, G Elbers, SR Lestari, M Diantoro, N Mufti, CS Ng, NANN Malek, Sunaryono, Y-H Chiu, S Zubaidah, Aulanni'am, I Wibowo & AY Handaya (eds), 4th International Conference on Life Science and Technology, ICoLiST 2023., 020032, AIP Conference Proceedings, vol. 2634, American Institute of Physics Inc., 4th International Conference on Life Science and Technology, ICoLiST 2023, Virtual, Online, Indonesia, 31/08/21. https://doi.org/10.1063/5.0117826

The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat. / Kurniawati, Elisa Danik; Lestary, Tanti Tri; Haryati, Ni Putu Sri et al.
4th International Conference on Life Science and Technology, ICoLiST 2023. ed. / Ahmad Taufiq; Hendra Susanto; I. Ching Wang; Hadi Nur; Muhammad Aziz; Chuang-Rung Chang; Gereon Elbers; Sri Rahayu Lestari; Markus Diantoro; Nandang Mufti; Chen Siang Ng; Nik Ahmad Nizam Nik Malek; Sunaryono; Yu-Hsin Chiu; Siti Zubaidah; Aulanni'am; Indra Wibowo; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2023. 020032 (AIP Conference Proceedings; Vol. 2634).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat

AU - Kurniawati, Elisa Danik

AU - Lestary, Tanti Tri

AU - Haryati, Ni Putu Sri

AU - Norahmawati, Eviana

AU - Wiyasa, I. Wayan Arsana

AU - Nurseta, Tatit

AU - Hidayati, Dwi Yuni

AU - Fitri, Loeki Enggar

PY - 2023/1/24

Y1 - 2023/1/24

N2 - Menopause can lead to health problems such as osteoporosis, neurological and cardiovascular disease. Neurologically, Alzheimer's is caused by the accumulation of amyloid peptides that form plaques in the brain resulting in neuronal death. In addition, the incidence of osteoporosis also increases during menopause which is indicated by an increase in bone RANKL expression. Nitric Oxide (NO) is a modulator of endothelial cell permeability, which shows an association with cardiovascular diseases. Genistein in cowpea is expected to have a protective effect. The study aims to prove that cowpea can reduce brain amyloid β, bone RANKL expression, NO levels of an ovariectomized rat. Twenty-four Rattus norvegicus females were divided into a control group (SHAM), an OVX group given 17-estradiol 0.18 mg/kg BW/day, and three OVX groups given extracts 1.25, 2.5, and 5 mg/kg. BB/day for 40 days. Brain amyloid β expression was measured by immunofluorescence, bone RANKL expression was observed by immunohistochemical staining, and NO levels by colorimetry method. Cowpea extract can be used as an antioxidant agent and was shown to significantly reduce the expression of brain amyloid β (p value 0.000), bone RANKL (p value 0.006), and no significant difference for NO levels (p value 0.162) in ovariectomized rats.

AB - Menopause can lead to health problems such as osteoporosis, neurological and cardiovascular disease. Neurologically, Alzheimer's is caused by the accumulation of amyloid peptides that form plaques in the brain resulting in neuronal death. In addition, the incidence of osteoporosis also increases during menopause which is indicated by an increase in bone RANKL expression. Nitric Oxide (NO) is a modulator of endothelial cell permeability, which shows an association with cardiovascular diseases. Genistein in cowpea is expected to have a protective effect. The study aims to prove that cowpea can reduce brain amyloid β, bone RANKL expression, NO levels of an ovariectomized rat. Twenty-four Rattus norvegicus females were divided into a control group (SHAM), an OVX group given 17-estradiol 0.18 mg/kg BW/day, and three OVX groups given extracts 1.25, 2.5, and 5 mg/kg. BB/day for 40 days. Brain amyloid β expression was measured by immunofluorescence, bone RANKL expression was observed by immunohistochemical staining, and NO levels by colorimetry method. Cowpea extract can be used as an antioxidant agent and was shown to significantly reduce the expression of brain amyloid β (p value 0.000), bone RANKL (p value 0.006), and no significant difference for NO levels (p value 0.162) in ovariectomized rats.

UR - https://www.scopus.com/pages/publications/85147266099

U2 - 10.1063/5.0117826

DO - 10.1063/5.0117826

M3 - Conference contribution

AN - SCOPUS:85147266099

T3 - AIP Conference Proceedings

BT - 4th International Conference on Life Science and Technology, ICoLiST 2023

A2 - Taufiq, Ahmad

A2 - Susanto, Hendra

A2 - Wang, I. Ching

A2 - Nur, Hadi

A2 - Aziz, Muhammad

A2 - Chang, Chuang-Rung

A2 - Elbers, Gereon

A2 - Lestari, Sri Rahayu

A2 - Diantoro, Markus

A2 - Mufti, Nandang

A2 - Ng, Chen Siang

A2 - Malek, Nik Ahmad Nizam Nik

A2 - Sunaryono, null

A2 - Chiu, Yu-Hsin

A2 - Zubaidah, Siti

A2 - Aulanni'am, null

A2 - Wibowo, Indra

A2 - Handaya, Adeodatus Yuda

PB - American Institute of Physics Inc.

T2 - 4th International Conference on Life Science and Technology, ICoLiST 2023

Y2 - 31 August 2021

ER -

Kurniawati ED, Lestary TT, Haryati NPS, Norahmawati E, Wiyasa IWA, Nurseta T et al. The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat. In Taufiq A, Susanto H, Wang IC, Nur H, Aziz M, Chang CR, Elbers G, Lestari SR, Diantoro M, Mufti N, Ng CS, Malek NANN, Sunaryono, Chiu YH, Zubaidah S, Aulanni'am, Wibowo I, Handaya AY, editors, 4th International Conference on Life Science and Technology, ICoLiST 2023. American Institute of Physics Inc. 2023. 020032. (AIP Conference Proceedings). doi: 10.1063/5.0117826

The Potency of Cowpea (Vigna unguiculata) as Antioxidant Agent in Ovariectomized Rat

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