The Molecular detection of blaVIM in carbapenem-resistant Acinetobacter baumannii-calcoaceticus complex infections in Indonesia

Background: Bacteria of carbapenem-resistant Acinetobacter baumannii-calcoaceticus complex (CRABC) is a significant pathogen causes illnesses acquired via healthcare around the world, however little is known regarding the molecular resistant gene of A. baumannii-calcoaceticus complex bacteria resistant to carbapenem in Indonesian healthcare facilities. This study is aimed to identify the carbapenemase resistant gene of CRABC in two referral care hospitals in the islands of North Sulawesiand East Java, Indonesia. Methods: The Vitek2® system was used to identify the CRABC pathogen that were obtained from standard medical cultures, which included blood, lower respiratory tract, wound and urine samples from patients treated in the medical wards, intensive care unit (ICU), surgical wards, neonatal intensive care unit (NICU), and cardiovascular intensive care unit (CVICU) in two referral care hospitals in Manado, North Sulawesi, and Malang, East Java. Using PCR, we identified the presence of the carbapenemase resistance genes bla_GES, bla_VIM, bla_IMP1, and bla_OXA-48 carbapenemase resistance genes. Results: Seventy threeA. baumannii-calcoaceticus complex carbapenem resistance bacteria were collected. The specimens from the lower respiratory tract specimens, the isolates of the carbapenem-resistant A. baumannii-calcoaceticus complex were frequently found.In this study, we detected the bla_VIM carbapenemase resistance genes from blood and sputum samples in tertiary care hospital in Malang. Conclusions: We identified the bla_VIM carbapenemase resistance genes were found in the referral care hospitals in Malang, East Java, Indonesia. Awareness of the national prevention and surveillance system is isolated by the prevention and reduction of CRABC transmission.