The effects of Eucheuma cottonii on alveolar macrophages and malondialdehyde levels in bronchoalveolar lavage fluid in chronically particulate matter 10 coal dust-exposed rats

Objective(s): To investigate the effect of Eucheuma cottonii on alveolar macrophages (AM) and malondialdehyde (MDA) levels in bronchoalveolar lavage fluids (BALF) in particulate matter 10 (PM₁₀) coal dust-exposed rats. Materials and Methods: Ten groups, including a non exposed group and groups exposed to coal dust at doses of 6.25 (CD_6.25), 12.5 (CD_12.5), or 25 mg/m3 (CD₂₅) an hour daily for 6 months with or without supplementation of ethanolic extract of E. cottonii at doses of 150 (EC₁₅₀) or 300 mg/kg BW (EC₃₀₀). The number of macrophages was determined using a light microscope. MDA levels were measured by TBARS assay. Results: EC₁₅₀ insignificantly (P > 0.05) reduces the AM in CD groups compared to non treatment groups. EC₁₅₀ and EC₃₀₀ significantly (P < 0.05) decreased MDA levels in CD_12.5 and CD₂₅ groups relative to non treatment groups.Conclusion: E. cottonii attenuated oxidative stress in chronic exposure of PM₁₀ coal dust.