The Effects of Equilibration Time and Post-Thawing Temperatures in Cryopreservation of Gaga Chicken Semen

This study aimed to evaluation the effect of different equilibration time and post thawing cryopreservation in gaga chicken semen. The research material was 10 male Gaga chickens who were 10 months old, healthy, and normal reproductive organs. Semen was collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh semen was evaluated macroscopically and microscopically. The sperm used had a motility of over 80%. The semen was diluted Lactate Ringer with 10% egg yolk and the addition of 7% DMSO cryoprotectant. Then the semen was equilibrated at different times and then frozen. The frozen semen was then stored in a liquid nitrogen container at a temperature of -196°C.After 24 hours, the semen was thawing at a different temperature. The evaluation of semen quality included motility, viability, plasma membrane integrity, and acrosome integrity. All data obtained were analyzed with two wayanalysis of variance. The result showed that equilibration time treatment and differential temperature of thawing yield good results on the motility and viability of Gaga chicken sperm on all treatments (P>0,01). Equilibration time of treatment for 2 hours was capable of preserving Plasma Membrane Integrity (PMI) and acrosomal integrity with a thawing temperature of 38 °C for 30 seconds or a 60 °C Thawing temperature for 5 seconds (P<0.01).