The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L.

Retno Mastuti; Aminatun Munawarti; Mufidatur Rosyidah

doi:10.1063/1.5012720

The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L.

Retno Mastuti^*
, Aminatun Munawarti
, Mufidatur Rosyidah

^*Corresponding author for this work

Faculty of Mathematics and Natural Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

5 Citations (Scopus)

Abstract

Physalis angulata L. (Ciplukan) which belongs to Solanaceae is an important medicinal plant. In vitro culture medium contains carbon source, inorganic substance, vitamins, and plant growth regulators. However, organic growth supplements have frequently been added to improve regeneration capability of explants. This study was conducted to observe the effect of tomato juices and extract bean sprout on shoot regeneration and multiplication of in vitro nodal explants. The explants were cultured on MS basal medium + 6-benzyl amino purine (BAP) 2 mg/L + indole-3-acetic acid (IAA) 0.05 mg/L with and without organic supplements. Tomato juices (T) 5, 7.5 and 10% or bean sprout extract (B) 1.25, 2.5, and 3.75% were added as natural organic supplements. Almost all explants have produced shoots one week after culture. After six weeks of culture maximum shoot number (12.5±3.9) was produced in medium MS + T5 while maximum shoot length (10.7 ± 0.7 cm) was obtained in medium MS + T 7.5. Medium T tends to produce more shoots than the medium B and medium control. This result indicates the potential of natural organic supplements for supporting Ciplukan propagation through in vitro culture.

Original language	English
Title of host publication	8th International Conference on Global Resource Conservation, ICGRC 2017
Subtitle of host publication	Green Campus Movement for Global Conservation
Editors	Akira Kikuchi, Chandrakhant Salunkhe, Dian Siswanto, Yoga Dwi Jatmiko, Bhupal Govinda
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735416000
DOIs	https://doi.org/10.1063/1.5012720
Publication status	Published - 29 Nov 2017
Event	8th International Conference on Global Resource Conservation: Green Campus Movement for Global Conservation, ICGRC 2017 - Malang, Indonesia Duration: 19 Jul 2017 → 20 Jul 2017

Publication series

Name	AIP Conference Proceedings
Volume	1908
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	8th International Conference on Global Resource Conservation: Green Campus Movement for Global Conservation, ICGRC 2017
Country/Territory	Indonesia
City	Malang
Period	19/07/17 → 20/07/17

Keywords

in vitro culture
medicinal plants
Physalis angulata L.
sprout bean extracts
tomato juices

Access to Document

10.1063/1.5012720

Cite this

Mastuti, R., Munawarti, A., & Rosyidah, M. (2017). The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L. In A. Kikuchi, C. Salunkhe, D. Siswanto, Y. D. Jatmiko, & B. Govinda (Eds.), 8th International Conference on Global Resource Conservation, ICGRC 2017: Green Campus Movement for Global Conservation Article 040006 (AIP Conference Proceedings; Vol. 1908). American Institute of Physics Inc.. https://doi.org/10.1063/1.5012720

Mastuti, Retno ; Munawarti, Aminatun ; Rosyidah, Mufidatur. / The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L. 8th International Conference on Global Resource Conservation, ICGRC 2017: Green Campus Movement for Global Conservation. editor / Akira Kikuchi ; Chandrakhant Salunkhe ; Dian Siswanto ; Yoga Dwi Jatmiko ; Bhupal Govinda. American Institute of Physics Inc., 2017. (AIP Conference Proceedings).

@inproceedings{66b23007c09d4b0e9dc780ee186052a5,

title = "The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L.",

abstract = "Physalis angulata L. (Ciplukan) which belongs to Solanaceae is an important medicinal plant. In vitro culture medium contains carbon source, inorganic substance, vitamins, and plant growth regulators. However, organic growth supplements have frequently been added to improve regeneration capability of explants. This study was conducted to observe the effect of tomato juices and extract bean sprout on shoot regeneration and multiplication of in vitro nodal explants. The explants were cultured on MS basal medium + 6-benzyl amino purine (BAP) 2 mg/L + indole-3-acetic acid (IAA) 0.05 mg/L with and without organic supplements. Tomato juices (T) 5, 7.5 and 10\% or bean sprout extract (B) 1.25, 2.5, and 3.75\% were added as natural organic supplements. Almost all explants have produced shoots one week after culture. After six weeks of culture maximum shoot number (12.5±3.9) was produced in medium MS + T5 while maximum shoot length (10.7 ± 0.7 cm) was obtained in medium MS + T 7.5. Medium T tends to produce more shoots than the medium B and medium control. This result indicates the potential of natural organic supplements for supporting Ciplukan propagation through in vitro culture.",

keywords = "in vitro culture, medicinal plants, Physalis angulata L., sprout bean extracts, tomato juices",

author = "Retno Mastuti and Aminatun Munawarti and Mufidatur Rosyidah",

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publisher = "American Institute of Physics Inc.",

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Mastuti, R , Munawarti, A & Rosyidah, M 2017, The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L. in A Kikuchi, C Salunkhe, D Siswanto, YD Jatmiko & B Govinda (eds), 8th International Conference on Global Resource Conservation, ICGRC 2017: Green Campus Movement for Global Conservation., 040006, AIP Conference Proceedings, vol. 1908, American Institute of Physics Inc., 8th International Conference on Global Resource Conservation: Green Campus Movement for Global Conservation, ICGRC 2017, Malang, Indonesia, 19/07/17. https://doi.org/10.1063/1.5012720

The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L. / Mastuti, Retno ; Munawarti, Aminatun; Rosyidah, Mufidatur.
8th International Conference on Global Resource Conservation, ICGRC 2017: Green Campus Movement for Global Conservation. ed. / Akira Kikuchi; Chandrakhant Salunkhe; Dian Siswanto; Yoga Dwi Jatmiko; Bhupal Govinda. American Institute of Physics Inc., 2017. 040006 (AIP Conference Proceedings; Vol. 1908).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L.

AU - Mastuti, Retno

AU - Munawarti, Aminatun

AU - Rosyidah, Mufidatur

PY - 2017/11/29

Y1 - 2017/11/29

N2 - Physalis angulata L. (Ciplukan) which belongs to Solanaceae is an important medicinal plant. In vitro culture medium contains carbon source, inorganic substance, vitamins, and plant growth regulators. However, organic growth supplements have frequently been added to improve regeneration capability of explants. This study was conducted to observe the effect of tomato juices and extract bean sprout on shoot regeneration and multiplication of in vitro nodal explants. The explants were cultured on MS basal medium + 6-benzyl amino purine (BAP) 2 mg/L + indole-3-acetic acid (IAA) 0.05 mg/L with and without organic supplements. Tomato juices (T) 5, 7.5 and 10% or bean sprout extract (B) 1.25, 2.5, and 3.75% were added as natural organic supplements. Almost all explants have produced shoots one week after culture. After six weeks of culture maximum shoot number (12.5±3.9) was produced in medium MS + T5 while maximum shoot length (10.7 ± 0.7 cm) was obtained in medium MS + T 7.5. Medium T tends to produce more shoots than the medium B and medium control. This result indicates the potential of natural organic supplements for supporting Ciplukan propagation through in vitro culture.

AB - Physalis angulata L. (Ciplukan) which belongs to Solanaceae is an important medicinal plant. In vitro culture medium contains carbon source, inorganic substance, vitamins, and plant growth regulators. However, organic growth supplements have frequently been added to improve regeneration capability of explants. This study was conducted to observe the effect of tomato juices and extract bean sprout on shoot regeneration and multiplication of in vitro nodal explants. The explants were cultured on MS basal medium + 6-benzyl amino purine (BAP) 2 mg/L + indole-3-acetic acid (IAA) 0.05 mg/L with and without organic supplements. Tomato juices (T) 5, 7.5 and 10% or bean sprout extract (B) 1.25, 2.5, and 3.75% were added as natural organic supplements. Almost all explants have produced shoots one week after culture. After six weeks of culture maximum shoot number (12.5±3.9) was produced in medium MS + T5 while maximum shoot length (10.7 ± 0.7 cm) was obtained in medium MS + T 7.5. Medium T tends to produce more shoots than the medium B and medium control. This result indicates the potential of natural organic supplements for supporting Ciplukan propagation through in vitro culture.

KW - in vitro culture

KW - medicinal plants

KW - Physalis angulata L.

KW - sprout bean extracts

KW - tomato juices

UR - https://www.scopus.com/pages/publications/85038942096

U2 - 10.1063/1.5012720

DO - 10.1063/1.5012720

M3 - Conference contribution

AN - SCOPUS:85038942096

T3 - AIP Conference Proceedings

BT - 8th International Conference on Global Resource Conservation, ICGRC 2017

A2 - Kikuchi, Akira

A2 - Salunkhe, Chandrakhant

A2 - Siswanto, Dian

A2 - Jatmiko, Yoga Dwi

A2 - Govinda, Bhupal

PB - American Institute of Physics Inc.

T2 - 8th International Conference on Global Resource Conservation: Green Campus Movement for Global Conservation, ICGRC 2017

Y2 - 19 July 2017 through 20 July 2017

ER -

Mastuti R , Munawarti A, Rosyidah M. The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L. In Kikuchi A, Salunkhe C, Siswanto D, Jatmiko YD, Govinda B, editors, 8th International Conference on Global Resource Conservation, ICGRC 2017: Green Campus Movement for Global Conservation. American Institute of Physics Inc. 2017. 040006. (AIP Conference Proceedings). doi: 10.1063/1.5012720

The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L.

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