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The Effect of Temperature on Survival Rate of White leg Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus

  • Irvan Firman Syah Za
  • , Azam Bachur Zaidy
  • , Ketut Sugama
  • , Soebhakti Hasan
  • , Ren Fitriadi*
  • , Muhammad Ikhwan Ihtifazhuddin
  • , Sata Yoshida Srie Rahayu
  • , I. Made Dedi Mahariawan
  • , K. kamaruddin
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Infectious myonecrosis virus (IMNV) is a significant pathogen in shrimp aquaculture, causing high mortality. Environmental factors, particularly temperature fluctuations, are known to trigger IMNV outbreaks. The present study aimed to determine the effects of hyperthermia on the survival rate and incubation period of whiteleg shrimp (Litopenaeus vannamei) infected with IMNV. The present study utilized a completely randomized design with five treatment groups and three replications. Each replication consisted of 15 shrimp, with an average weight of 9.19 ±0.58 grams, reared in 100 L plastic containers filled with seawater at 26 ppt salinity. The treatment groups consisted of shrimp infected with IMNV via intramuscular injection and reared at temperatures of 30°C (S30, positive control group), 31°C (S31), 32°C (S32), and 33°C (S33). Additionally, a negative control group of non-infected shrimp was reared at 30°C (Sk30). The observation period lasted 10 days post-infection. The present results demonstrated that hyperthermia significantly impacted shrimp mortality and viral incubation. The highest survival rate was observed in the control group at 97.78. Among the infected groups, the highest survival rates were recorded in S30 (51.11) and S31 (48.89), whereas the lowest were in S32 (28.89) and S33 (24.44). In the S33 treatment, higher temperatures reduced the incubation period to 2 days, whereas in the S30 treatment, it lasted for 5 days. The current findings indicated that water temperatures of 32°C and 33°C act as environmental stressors, significantly reducing IMNV incubation period and increasing the risk of mortality in Litopenaeus vannamei shrimp.

Original languageEnglish
Pages (from-to)32-42
Number of pages11
JournalWorld's Veterinary Journal
Volume16
Issue number1
DOIs
Publication statusPublished - Mar 2026

Keywords

  • Hyperthermia
  • Incubation period
  • Infectious myonecrosis virus
  • Survival rate

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