TY - GEN
T1 - The Effect of Soy Milk on Estrogen Receptor Alpha Expression in Medial Preoptic Area (MPOA) and in Spermatogonia, Testosterone Levels, and Androgen Receptors Expression in Male Wistar Rats (Rattus norvegicus)
AU - Nurdiana, Nurdiana
AU - Chania, Made Pradnyawati
AU - Nurvitasari, Rifzi Devi
AU - Nisa, Azmiatun
AU - Diana, Styan Wahyu
AU - Rochmah, Evalina Izzatur
AU - Mayangsari, Elly
AU - Rahardjo, Bambang
AU - Indrawan, I. Wayan Agung
AU - Khotimah, Husnul
AU - Mintaroem, Karyono
AU - Norahmawati, Eviana
AU - Raras, Tri Yudani Mardining
N1 - Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/1/24
Y1 - 2023/1/24
N2 - Soy milk contains high isoflavones that have biological activity similar to estrogen, capable of binding to a estrogen receptors, and is often referred to as phytoestrogens which can disrupt endocrine. ER is found in several mammalian tissues, including the reproductive tract. This study aims to analyze the effect of soy milk on ERa expression in MPOA and in spermatogonia cells, testosterone levels, and AR expression in testes. Twenty-four male Wistar rats (Rattus norvegicus) were divided into four groups: control group (given nonnal diet) and PI; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively, for 6 weeks). IHC method was used to analyze the expression of ERa in MPOA and spermatogonia cells, and AR in testes. Testosterone levels were measured by ELISA. The results showed that the expression of ERa in MPOA and spermatogonia cells, AR expression in the testes, and testosterone levels were lower in treatment groups than in the control group. Soy milk exposure in male rats can affect the regulation of receptor expression and estrogen levels in the male reproductive system which may be related to endocrine disrupter processes and infertility.
AB - Soy milk contains high isoflavones that have biological activity similar to estrogen, capable of binding to a estrogen receptors, and is often referred to as phytoestrogens which can disrupt endocrine. ER is found in several mammalian tissues, including the reproductive tract. This study aims to analyze the effect of soy milk on ERa expression in MPOA and in spermatogonia cells, testosterone levels, and AR expression in testes. Twenty-four male Wistar rats (Rattus norvegicus) were divided into four groups: control group (given nonnal diet) and PI; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively, for 6 weeks). IHC method was used to analyze the expression of ERa in MPOA and spermatogonia cells, and AR in testes. Testosterone levels were measured by ELISA. The results showed that the expression of ERa in MPOA and spermatogonia cells, AR expression in the testes, and testosterone levels were lower in treatment groups than in the control group. Soy milk exposure in male rats can affect the regulation of receptor expression and estrogen levels in the male reproductive system which may be related to endocrine disrupter processes and infertility.
UR - https://www.scopus.com/pages/publications/85147300831
U2 - 10.1063/5.0111499
DO - 10.1063/5.0111499
M3 - Conference contribution
AN - SCOPUS:85147300831
T3 - AIP Conference Proceedings
BT - 4th International Conference on Life Science and Technology, ICoLiST 2023
A2 - Taufiq, Ahmad
A2 - Susanto, Hendra
A2 - Wang, I. Ching
A2 - Nur, Hadi
A2 - Aziz, Muhammad
A2 - Chang, Chuang-Rung
A2 - Elbers, Gereon
A2 - Lestari, Sri Rahayu
A2 - Diantoro, Markus
A2 - Mufti, Nandang
A2 - Ng, Chen Siang
A2 - Malek, Nik Ahmad Nizam Nik
A2 - Sunaryono, null
A2 - Chiu, Yu-Hsin
A2 - Zubaidah, Siti
A2 - Aulanni'am, null
A2 - Wibowo, Indra
A2 - Handaya, Adeodatus Yuda
PB - American Institute of Physics Inc.
T2 - 4th International Conference on Life Science and Technology, ICoLiST 2023
Y2 - 31 August 2021
ER -