TY - GEN
T1 - The Effect of Mango Mistletoes (Dendrophthoe pentandra) Leaves Extract toward Immunosenescence on Old Balb/c Mice
AU - Handono, Kusworini
AU - Pratama, Mirza Zaka
AU - Yuniati, Maria Gabriela
AU - Sermoati, Inmas Andi
AU - Norahmawati, Eviana
AU - Endharti, Agustina Tri
AU - Irwanto, Yahya
AU - Hidayat, Syaiful
AU - Solikhin, Muhammad Badrus
AU - Indahwati, Lilik
AU - Fatmawati, Fatmawati
N1 - Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/1/24
Y1 - 2023/1/24
N2 - Immunosenescence triggers increased morbidity in geriatrics; therefore, therapeutic intervention is required. This study aims to find out the effect of mango mistletoes/Dendrophthoe pentandra (MM/Dp) towards CD4/CD8 ratio, CD4+CD28" percentage, CD8+CD28" percentage, and IL-2 level on old female mice. The mango mistletoes leaves were extracted using ethanol 96%, and then administered orally once a day for 30 days (150, 300, and 600 mg/kgBW). CD4/CD8 ratio, and percentage of CD4+CD28" and CD8+CD28" were examined using flowcytometry on the spleen sample. ELISA was performed on the blood sample extracted from the heart to study the IL-2 level. MM/Dp could lower the percentage of CD4+CD28- significantly (p = 0.001) (300 and 600 mg/kgBW), but statistically insignificant (p = 0.121) in reducing the percentage of CD8+CD28" (but has positive trend). Although MM/Dp was insignificant in increasing the IL-2 level (p = 0.124), there was an increase in trend at a dose of 600 mg/kgBW (2.53 pg/ml) compared to the control group (2.02 pg/ml). The administration of various doses had a strong correlation (r = 0.496) with the decreasing CD4/CD8 ratio. MM/Dp has potentials in inhibiting immunosenescence that is signified by the decreasing percentage of CD4+CD28" and CD8+CD28", as well as increasing the IL-2 level.
AB - Immunosenescence triggers increased morbidity in geriatrics; therefore, therapeutic intervention is required. This study aims to find out the effect of mango mistletoes/Dendrophthoe pentandra (MM/Dp) towards CD4/CD8 ratio, CD4+CD28" percentage, CD8+CD28" percentage, and IL-2 level on old female mice. The mango mistletoes leaves were extracted using ethanol 96%, and then administered orally once a day for 30 days (150, 300, and 600 mg/kgBW). CD4/CD8 ratio, and percentage of CD4+CD28" and CD8+CD28" were examined using flowcytometry on the spleen sample. ELISA was performed on the blood sample extracted from the heart to study the IL-2 level. MM/Dp could lower the percentage of CD4+CD28- significantly (p = 0.001) (300 and 600 mg/kgBW), but statistically insignificant (p = 0.121) in reducing the percentage of CD8+CD28" (but has positive trend). Although MM/Dp was insignificant in increasing the IL-2 level (p = 0.124), there was an increase in trend at a dose of 600 mg/kgBW (2.53 pg/ml) compared to the control group (2.02 pg/ml). The administration of various doses had a strong correlation (r = 0.496) with the decreasing CD4/CD8 ratio. MM/Dp has potentials in inhibiting immunosenescence that is signified by the decreasing percentage of CD4+CD28" and CD8+CD28", as well as increasing the IL-2 level.
UR - https://www.scopus.com/pages/publications/85147299240
U2 - 10.1063/5.0117337
DO - 10.1063/5.0117337
M3 - Conference contribution
AN - SCOPUS:85147299240
T3 - AIP Conference Proceedings
BT - 4th International Conference on Life Science and Technology, ICoLiST 2023
A2 - Taufiq, Ahmad
A2 - Susanto, Hendra
A2 - Wang, I. Ching
A2 - Nur, Hadi
A2 - Aziz, Muhammad
A2 - Chang, Chuang-Rung
A2 - Elbers, Gereon
A2 - Lestari, Sri Rahayu
A2 - Diantoro, Markus
A2 - Mufti, Nandang
A2 - Ng, Chen Siang
A2 - Malek, Nik Ahmad Nizam Nik
A2 - Sunaryono, null
A2 - Chiu, Yu-Hsin
A2 - Zubaidah, Siti
A2 - Aulanni'am, null
A2 - Wibowo, Indra
A2 - Handaya, Adeodatus Yuda
PB - American Institute of Physics Inc.
T2 - 4th International Conference on Life Science and Technology, ICoLiST 2023
Y2 - 31 August 2021
ER -