The effect of ginger soy milk (Sulehe) combination on histopathology of pancreas and muscle of mouse model of insulin resistance

Type 2 Diabetes Mellitus (T2DM) is a metabolic disease characterized by chronic hyperglycemia and hyperinsulinemia. T2DM is closely associated with the condition of insulin resistance. Insulin resistance is associated with the presence of hyperinsulinemia characterized by a decreased tyrosine kinase enzyme activity and an increased tyrosine phosphatase enzyme activity. One alternative attempt to overcome insulin resistance in T2DM is the administration of soy-based nutrition. Soy milk is one of the processed soybeans containing polysaccharide protein, carbohydrate, fat, vitamin, mineral, fiber, isoflavones and low glycemic index (GI). This study aimed to determine the effect of ginger soymilk (Sulehe) combination on histopathology of pancreas and muscle of mouse model of insulin resistance. This study used true experimental laboratories with Post-Test Only Control Group Design. Selection of subjects for grouping and treatment was done using randomization with 2 control groups, i.e. negative control (pap milk and aquadest) and positive control (DTLF) and 4 treatment groups, i.e. P1 (positive control + soy milk 5000 mg/day), P2 (positive control + ginger 500 mg/kgBW), P3 (positive control + ginger soy milk 2500:250 mg/kgBW) and P4 (positive control + ginger soy milk 5000:500 mg/kgBW). Histopathology of pancreas and muscle was analyzed using HE staining and immunohistochemistry methods. This study used mouse model of insulin resistance induced by a combination of High Fat and Fructose Diet (DTLF) for 16 weeks and low dose of STZ 25 mg/kgBW. The study was approved by the Health Research Ethics Committee No. 687-KEP-UB. The results showed that in the ginger soy milk (sulehe) group, particularly combination II (5000:500 mg/kgBW), was evident to improve the histology of pancreas and muscle to the normal state.