The Effect of Different Tofu Liquid Waste Doses on Decreasing Alveolar Bone Resorption in Rats

Introduction: P. gingivalis are the main bacteria that cause periodontal diseases. One of the clinical signs of periodontal disease is alveolar bone resorption. Tofu liquid waste is a typical high water-containing biomass and isoflavones. Isoflavones is a polyphenol that has an anti-inflammatory effect by reducing pro-inflammatory cytokine expression. This study aims to evaluate whether systemic administration of different tofu liquid waste doses has effects on decreasing alveolar bone resorption of the Wistar rats' periodontal tissues induced by P. gingivalis. Materials and methods: : This is true experimental research, with a randomized posttest-only group design. The groups are K (-) for the normal group, K (+) for the negative control group, and K-1, K-2, and K-3 for group induced with P.gingivalis [0.03 mL(1x 109CFU/mL)] and administrated with 6, 12, and 18 mL/kgBW tofu liquid waste respectively every day for 28 days. Results: Freeze-dried technique result showed tofu liquid waste still contains isoflavone (daidzin and glycitin). There is no difference in mean in all parameters between K(-) and K-3. K(+) has difference mean with K-1, K-2, and K-3. Correlation test showed there was a positive correlation between the dose levels of tofu liquid waste with osteoblasts and OPG, and a negative correlation between the dose levels of tofu liquid waste with lymphocytes, neutrophils, osteoclasts, RANKL, alveolar bone resorption, and periodontal ligament space. Conclusion: Tofu liquid waste administration has effects on decreasing the alveolar bone resorption of Wistar rat (Rattus norvegicus) periodontal tissues induced by P. gingivalis and 18 mL/kgBW has the highest effect.