The effect of commercial nutrients to increase the population of Skeletonema costatum on laboratory and mass scales

S. costatum is one type of phytoplankton used in the maintenance of fish, shrimp, and crab larvae, but most are given in the maintenance of tiger shrimp larvae (Penaeus monodon) from the phase of nauplius phase after zoea. S. costatum has the advantages found in the autolysis enzyme itself so that it is easily digested by the larvae and does not pollute the cultivation media. Therefore knowledge about how is the effect of nutrients in S. costatum commercial life of food culture on laboratory scale up to mass scale. The method S. costatum is generally started by sterilizing tools and materials, preparation of culture media for laboratory scale (2 liters), semi-mass scale (80 liters) and mass scale (2 tons), fertilizer application, seed selection, the cost of counting S. costatum cells with peak population depends on the 36th hour with the highest number of cells in each culture scale items, namely laboratory scale of 602.547 × 10³ cells/mL, semi-mass scale of 484.713 × 10³ cells/mL and mass scale of 133.757 × 103 cells/mL then the last process is harvesting until it becomes flour. The obstacles encountered are environmental problems such as unstable weather and can be contaminated.