The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus

Eka Frenty Hadiningsih; Mayasari Putri Ardela; S. Suryanti; Tatit Nurseta; Noorhamdani; Sri Winarsih; Kenty Wantri Anita; Aina Angelina

doi:10.1063/5.0002908

The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus

Eka Frenty Hadiningsih^*
, Mayasari Putri Ardela
, S. Suryanti
, Tatit Nurseta
, Noorhamdani
, Sri Winarsih
, Kenty Wantri Anita
, Aina Angelina

^*Corresponding author for this work

Faculty of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

DMPA is a type of hormonal contraceptive containing progesterone that causes a hypoestrogenic response that inhibits follicle development, prevents ovulation, and causes endometrial atrophy due to suppression of epithelial and stromal cell proliferation. Natural herbs like bengkuang can be used to reduce the negative effects of DMPA. This study aimed to determine the effect of bengkuang ethanol extract on the number of ovarian follicles, epithelium, and endometrial stroma in DMPA-treated Rattus norvegicus. This study used 25 rats as experimental animals, which were divided into five groups consisting of one negative control group without DMPA and bengkuang extract, and four treatment groups given DMPA every three days repeated four times. After the induction period, the hypoestrogenic condition was evaluated through vaginal smear. Three groups were treated by bengkuang extract. Data were analyzed using a one-way ANOVA and the Least Significant Difference (LSD) test. The results showed that there were significant differences (p< 0.05) in the number of antral follicles, the amount of the epithelium, and the amount of endometrial stroma. This study suggests that bengkuang extract demonstrated a change in the number of ovarian follicles, epithelium, and endometrial stroma compared to the positive control group treated only with DMPA.

Original language	English
Title of host publication	International Conference on Life Science and Technology, ICoLiST 2019
Editors	Ahmad Taufiq, Hendra Susanto, Hadi Nur, Muhammad Aziz, Chuang-Rung Chang, Hyohyemi Lee, Markus Diantoro, Nandang Mufti, Nik Ahmad Nizam Nik Malek, I. Ching Wang, Djoko Tjahnono Iskandar, Gereon Elbers, Sunaryono Sunaryono, Siti Zubaidah, Sumari Sumari, Aulanni'am, Asep Bayu Nandiyanto, Indra Wibowo, Adeodatus Yuda Handaya
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735419902
DOIs	https://doi.org/10.1063/5.0002908
Publication status	Published - 21 Apr 2020
Event	2nd International Conference on Life Science and Technology, ICoLiST 2019 - Malang, East Java, Indonesia Duration: 12 Sept 2019 → 13 Sept 2019

Publication series

Name	AIP Conference Proceedings
Volume	2231
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	2nd International Conference on Life Science and Technology, ICoLiST 2019
Country/Territory	Indonesia
City	Malang, East Java
Period	12/09/19 → 13/09/19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1063/5.0002908

Cite this

Hadiningsih, E. F., Ardela, M. P., Suryanti, S., Nurseta, T., Noorhamdani, Winarsih, S., Anita, K. W., & Angelina, A. (2020). The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus. In A. Taufiq, H. Susanto, H. Nur, M. Aziz, C.-R. Chang, H. Lee, M. Diantoro, N. Mufti, N. A. N. N. Malek, I. C. Wang, D. T. Iskandar, G. Elbers, S. Sunaryono, S. Zubaidah, S. Sumari, Aulanni'am, A. B. Nandiyanto, I. Wibowo, & A. Y. Handaya (Eds.), International Conference on Life Science and Technology, ICoLiST 2019 Article 2908 (AIP Conference Proceedings; Vol. 2231). American Institute of Physics Inc.. https://doi.org/10.1063/5.0002908

Hadiningsih, Eka Frenty ; Ardela, Mayasari Putri ; Suryanti, S. et al. / The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus. International Conference on Life Science and Technology, ICoLiST 2019. editor / Ahmad Taufiq ; Hendra Susanto ; Hadi Nur ; Muhammad Aziz ; Chuang-Rung Chang ; Hyohyemi Lee ; Markus Diantoro ; Nandang Mufti ; Nik Ahmad Nizam Nik Malek ; I. Ching Wang ; Djoko Tjahnono Iskandar ; Gereon Elbers ; Sunaryono Sunaryono ; Siti Zubaidah ; Sumari Sumari ; Aulanni'am ; Asep Bayu Nandiyanto ; Indra Wibowo ; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2020. (AIP Conference Proceedings).

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title = "The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus",

abstract = "DMPA is a type of hormonal contraceptive containing progesterone that causes a hypoestrogenic response that inhibits follicle development, prevents ovulation, and causes endometrial atrophy due to suppression of epithelial and stromal cell proliferation. Natural herbs like bengkuang can be used to reduce the negative effects of DMPA. This study aimed to determine the effect of bengkuang ethanol extract on the number of ovarian follicles, epithelium, and endometrial stroma in DMPA-treated Rattus norvegicus. This study used 25 rats as experimental animals, which were divided into five groups consisting of one negative control group without DMPA and bengkuang extract, and four treatment groups given DMPA every three days repeated four times. After the induction period, the hypoestrogenic condition was evaluated through vaginal smear. Three groups were treated by bengkuang extract. Data were analyzed using a one-way ANOVA and the Least Significant Difference (LSD) test. The results showed that there were significant differences (p< 0.05) in the number of antral follicles, the amount of the epithelium, and the amount of endometrial stroma. This study suggests that bengkuang extract demonstrated a change in the number of ovarian follicles, epithelium, and endometrial stroma compared to the positive control group treated only with DMPA.",

author = "Hadiningsih, \{Eka Frenty\} and Ardela, \{Mayasari Putri\} and S. Suryanti and Tatit Nurseta and Noorhamdani and Sri Winarsih and Anita, \{Kenty Wantri\} and Aina Angelina",

note = "Publisher Copyright: {\textcopyright} 2020 American Institute of Physics Inc.. All rights reserved.; 2nd International Conference on Life Science and Technology, ICoLiST 2019 ; Conference date: 12-09-2019 Through 13-09-2019",

year = "2020",

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day = "21",

doi = "10.1063/5.0002908",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Ahmad Taufiq and Hendra Susanto and Hadi Nur and Muhammad Aziz and Chuang-Rung Chang and Hyohyemi Lee and Markus Diantoro and Nandang Mufti and Malek, \{Nik Ahmad Nizam Nik\} and Wang, \{I. Ching\} and Iskandar, \{Djoko Tjahnono\} and Gereon Elbers and Sunaryono Sunaryono and Siti Zubaidah and Sumari Sumari and Aulanni'am and Nandiyanto, \{Asep Bayu\} and Indra Wibowo and Handaya, \{Adeodatus Yuda\}",

booktitle = "International Conference on Life Science and Technology, ICoLiST 2019",

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Hadiningsih, EF, Ardela, MP, Suryanti, S, Nurseta, T, Noorhamdani, Winarsih, S, Anita, KW & Angelina, A 2020, The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus. in A Taufiq, H Susanto, H Nur, M Aziz, C-R Chang, H Lee, M Diantoro, N Mufti, NANN Malek, IC Wang, DT Iskandar, G Elbers, S Sunaryono, S Zubaidah, S Sumari, Aulanni'am, AB Nandiyanto, I Wibowo & AY Handaya (eds), International Conference on Life Science and Technology, ICoLiST 2019., 2908, AIP Conference Proceedings, vol. 2231, American Institute of Physics Inc., 2nd International Conference on Life Science and Technology, ICoLiST 2019, Malang, East Java, Indonesia, 12/09/19. https://doi.org/10.1063/5.0002908

The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus. / Hadiningsih, Eka Frenty; Ardela, Mayasari Putri; Suryanti, S. et al.
International Conference on Life Science and Technology, ICoLiST 2019. ed. / Ahmad Taufiq; Hendra Susanto; Hadi Nur; Muhammad Aziz; Chuang-Rung Chang; Hyohyemi Lee; Markus Diantoro; Nandang Mufti; Nik Ahmad Nizam Nik Malek; I. Ching Wang; Djoko Tjahnono Iskandar; Gereon Elbers; Sunaryono Sunaryono; Siti Zubaidah; Sumari Sumari; Aulanni'am; Asep Bayu Nandiyanto; Indra Wibowo; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2020. 2908 (AIP Conference Proceedings; Vol. 2231).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus

AU - Hadiningsih, Eka Frenty

AU - Ardela, Mayasari Putri

AU - Suryanti, S.

AU - Nurseta, Tatit

AU - Noorhamdani,

AU - Winarsih, Sri

AU - Anita, Kenty Wantri

AU - Angelina, Aina

PY - 2020/4/21

Y1 - 2020/4/21

N2 - DMPA is a type of hormonal contraceptive containing progesterone that causes a hypoestrogenic response that inhibits follicle development, prevents ovulation, and causes endometrial atrophy due to suppression of epithelial and stromal cell proliferation. Natural herbs like bengkuang can be used to reduce the negative effects of DMPA. This study aimed to determine the effect of bengkuang ethanol extract on the number of ovarian follicles, epithelium, and endometrial stroma in DMPA-treated Rattus norvegicus. This study used 25 rats as experimental animals, which were divided into five groups consisting of one negative control group without DMPA and bengkuang extract, and four treatment groups given DMPA every three days repeated four times. After the induction period, the hypoestrogenic condition was evaluated through vaginal smear. Three groups were treated by bengkuang extract. Data were analyzed using a one-way ANOVA and the Least Significant Difference (LSD) test. The results showed that there were significant differences (p< 0.05) in the number of antral follicles, the amount of the epithelium, and the amount of endometrial stroma. This study suggests that bengkuang extract demonstrated a change in the number of ovarian follicles, epithelium, and endometrial stroma compared to the positive control group treated only with DMPA.

AB - DMPA is a type of hormonal contraceptive containing progesterone that causes a hypoestrogenic response that inhibits follicle development, prevents ovulation, and causes endometrial atrophy due to suppression of epithelial and stromal cell proliferation. Natural herbs like bengkuang can be used to reduce the negative effects of DMPA. This study aimed to determine the effect of bengkuang ethanol extract on the number of ovarian follicles, epithelium, and endometrial stroma in DMPA-treated Rattus norvegicus. This study used 25 rats as experimental animals, which were divided into five groups consisting of one negative control group without DMPA and bengkuang extract, and four treatment groups given DMPA every three days repeated four times. After the induction period, the hypoestrogenic condition was evaluated through vaginal smear. Three groups were treated by bengkuang extract. Data were analyzed using a one-way ANOVA and the Least Significant Difference (LSD) test. The results showed that there were significant differences (p< 0.05) in the number of antral follicles, the amount of the epithelium, and the amount of endometrial stroma. This study suggests that bengkuang extract demonstrated a change in the number of ovarian follicles, epithelium, and endometrial stroma compared to the positive control group treated only with DMPA.

UR - https://www.scopus.com/pages/publications/85092552753

U2 - 10.1063/5.0002908

DO - 10.1063/5.0002908

M3 - Conference contribution

AN - SCOPUS:85092552753

T3 - AIP Conference Proceedings

BT - International Conference on Life Science and Technology, ICoLiST 2019

A2 - Taufiq, Ahmad

A2 - Susanto, Hendra

A2 - Nur, Hadi

A2 - Aziz, Muhammad

A2 - Chang, Chuang-Rung

A2 - Lee, Hyohyemi

A2 - Diantoro, Markus

A2 - Mufti, Nandang

A2 - Malek, Nik Ahmad Nizam Nik

A2 - Wang, I. Ching

A2 - Iskandar, Djoko Tjahnono

A2 - Elbers, Gereon

A2 - Sunaryono, Sunaryono

A2 - Zubaidah, Siti

A2 - Sumari, Sumari

A2 - Aulanni'am, null

A2 - Nandiyanto, Asep Bayu

A2 - Wibowo, Indra

A2 - Handaya, Adeodatus Yuda

PB - American Institute of Physics Inc.

T2 - 2nd International Conference on Life Science and Technology, ICoLiST 2019

Y2 - 12 September 2019 through 13 September 2019

ER -

Hadiningsih EF, Ardela MP, Suryanti S, Nurseta T, Noorhamdani, Winarsih S et al. The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus. In Taufiq A, Susanto H, Nur H, Aziz M, Chang CR, Lee H, Diantoro M, Mufti N, Malek NANN, Wang IC, Iskandar DT, Elbers G, Sunaryono S, Zubaidah S, Sumari S, Aulanni'am, Nandiyanto AB, Wibowo I, Handaya AY, editors, International Conference on Life Science and Technology, ICoLiST 2019. American Institute of Physics Inc. 2020. 2908. (AIP Conference Proceedings). doi: 10.1063/5.0002908

The effect of Bengkuang (Pachyrhizus erosus) ethanol extract on the number of ovarian follicles, amount of epithelium and endometrium stroma cells in DMPA-treated rattus norvegicus

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