The effect of addition of glutathione in diluent Ringer's on spermatozoa quality of indigenous chicken during cold storage

Indigenous chicken is a local chicken of Indonesia which should be preserved as a local genetic resourcesbecause it has the advantage of high adaptability to the environment. This study aimed to determine the effect of the antioxidant glutathione in Ringer's diluent to on sperm quality of local chicken during storage at 3-5 °C. Maintenance and semen collection of indigenous chicken with female method performed at the College of Agricultural Extension(STPP) Malang. Semen obtained from collection was divided into 4 treatment that P0 (Ringer's without glutathione), P₁ (Ringer's+0.1 mM glutathione), P₂ (Ringer's + 0.5 mM glutathione) and P₃ (Ringer's + 1.0 mM glutathione) with a concentration 200x106 sperm cells/mL each treatment. Evaluation of semen quality during cold storage include individual motility, viability, and sperm abnormality was observed at hour 0, 4, 8, 24, 32 and 48. Randomized block design was used for the experiment and the data obtained were analized by Analysis of Variance (ANOVA). The result of theanalysis showed that theaddition of glutathione in the Ringer's diluent showed significant differences (P <0.05) on individual motility, viability and sperm abnormality in indigenous chicken. The best results are shown in the treatment of P₂ (Ringer's +0.5 mM glutathione). Sperm quality can be preserved in cold storage until 8 hours of P₂ treatment, with a mean of individual motility 62 ± 2.91%, viability 79.26 ± 2.68% and 13.66 ± 0.31% abnormality. The addition of 0.5 mM glutathione in Ringer's diluent and storage up to 8 hours at the temperature of 3-5 °C, produce quality requirements for use in artificial insemination. It is recommended that for further research to determine fertility and hatchability of the eggs obtained from female thatwere inseminated using semen diluted using Ringer's + 0.5 mM glutathione with cold storage up to 8 hours.