Skip to main navigation Skip to search Skip to main content

The effect of 0.6 and 0.8% Bovine Serum Albumin (BSA) levels in the Cauda Epididymal Plasma-2 (CEP-2) diluent to maintain Ongole Crossbred post-Thawing motility sperm

  • A. Rachmawati
  • , Ismaya
  • , B. P. Widyobroto
  • , S. Bintara
  • , T. Susilawati*
  • *Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

Abstract

Decreasing in post-Thawing sperm motility of Ongole Crossbred (OC) influences the success of conception through artificial insemination (AI). Sperm membrane damaged during frozen storage is assumed as the cause of a decrease in post-Thawing motility. Sperm motility is one of the critical indicators of frozen semen processing AI purposes. This study was aimed to assess the best BSA levels in Cauda Epididymal Plasma-2 (CEP-2) diluent in maintaining sperm post-Thawing motility. This study used fresh ejaculate of a three-year-old bull, collected once a week using artificial vagina. The experiment was based on BSA level 0; 0.6 and 0.8% in CEP-2+10% egg yolk. The results showed that different BSA levels in CEP-2+10% egg yolk diluents were able to maintain post-Thawing sperm motility: 42±2.58; 44±2.11, and 44±2.11% for control (0%), 0. 6% and 0. 8% BSA level, respectively. It is concluded that CEP-2+10% egg yolk + 0.6% BSA level effectively maintained sperm post-Thawing motility.

Original languageEnglish
Article number012034
JournalIOP Conference Series: Earth and Environmental Science
Volume387
Issue number1
DOIs
Publication statusPublished - 6 Dec 2019
Event8th International Seminar on Tropical Animal Production, ISTAP 2019 - Yogyakarta, Indonesia
Duration: 23 Sept 201925 Sept 2019

Fingerprint

Dive into the research topics of 'The effect of 0.6 and 0.8% Bovine Serum Albumin (BSA) levels in the Cauda Epididymal Plasma-2 (CEP-2) diluent to maintain Ongole Crossbred post-Thawing motility sperm'. Together they form a unique fingerprint.

Cite this