The complete amino acid sequence determination of a novel protein by mass spectrometry with the tentative human consensus sequence database

Ken Ichi Yoshino; Kenta Hara; Noriko Oshiro; Sujuti Hidayat; Chiharu Tokunaga; Kazuyoshi Yonezawa

The complete amino acid sequence determination of a novel protein by mass spectrometry with the tentative human consensus sequence database

Ken Ichi Yoshino^*
, Kenta Hara
, Noriko Oshiro
, Sujuti Hidayat
, Chiharu Tokunaga
, Kazuyoshi Yonezawa

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

A complete amino acid sequence of protein was determined by mass spectrometry with the tentative human consensus sequence (THC) database. To evaluate the advantages of THC database, it was applied for identification and in silico complete amino acid sequence determination of a novel protein p150, the mammalian target of rapamycin (mTOR)-associated protein. It was observed that expressed sequence tag (EST) database is a good source for protein identification and characterization in proteomics, even in the absence of entry in protein sequence database. The results show a complete amino acid sequencing of p150 by assembling both sequences of THC597050 and KIAA1303 in silico, which was confirmed by PCR and d^'-rapid amplification of cDNA ends.

Original language	English
Pages	751-752
Number of pages	2
Publication status	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

Cite this

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title = "The complete amino acid sequence determination of a novel protein by mass spectrometry with the tentative human consensus sequence database",

abstract = "A complete amino acid sequence of protein was determined by mass spectrometry with the tentative human consensus sequence (THC) database. To evaluate the advantages of THC database, it was applied for identification and in silico complete amino acid sequence determination of a novel protein p150, the mammalian target of rapamycin (mTOR)-associated protein. It was observed that expressed sequence tag (EST) database is a good source for protein identification and characterization in proteomics, even in the absence of entry in protein sequence database. The results show a complete amino acid sequencing of p150 by assembling both sequences of THC597050 and KIAA1303 in silico, which was confirmed by PCR and d'-rapid amplification of cDNA ends.",

author = "Yoshino, \{Ken Ichi\} and Kenta Hara and Noriko Oshiro and Sujuti Hidayat and Chiharu Tokunaga and Kazuyoshi Yonezawa",

year = "2002",

language = "English",

pages = "751--752",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Yoshino, KI, Hara, K, Oshiro, N, Hidayat, S, Tokunaga, C & Yonezawa, K 2002, 'The complete amino acid sequence determination of a novel protein by mass spectrometry with the tentative human consensus sequence database', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 2/06/02 - 6/06/02 pp. 751-752.

The complete amino acid sequence determination of a novel protein by mass spectrometry with the tentative human consensus sequence database. / Yoshino, Ken Ichi; Hara, Kenta; Oshiro, Noriko et al.
2002. 751-752 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - The complete amino acid sequence determination of a novel protein by mass spectrometry with the tentative human consensus sequence database

AU - Yoshino, Ken Ichi

AU - Hara, Kenta

AU - Oshiro, Noriko

AU - Hidayat, Sujuti

AU - Tokunaga, Chiharu

AU - Yonezawa, Kazuyoshi

PY - 2002

Y1 - 2002

N2 - A complete amino acid sequence of protein was determined by mass spectrometry with the tentative human consensus sequence (THC) database. To evaluate the advantages of THC database, it was applied for identification and in silico complete amino acid sequence determination of a novel protein p150, the mammalian target of rapamycin (mTOR)-associated protein. It was observed that expressed sequence tag (EST) database is a good source for protein identification and characterization in proteomics, even in the absence of entry in protein sequence database. The results show a complete amino acid sequencing of p150 by assembling both sequences of THC597050 and KIAA1303 in silico, which was confirmed by PCR and d'-rapid amplification of cDNA ends.

AB - A complete amino acid sequence of protein was determined by mass spectrometry with the tentative human consensus sequence (THC) database. To evaluate the advantages of THC database, it was applied for identification and in silico complete amino acid sequence determination of a novel protein p150, the mammalian target of rapamycin (mTOR)-associated protein. It was observed that expressed sequence tag (EST) database is a good source for protein identification and characterization in proteomics, even in the absence of entry in protein sequence database. The results show a complete amino acid sequencing of p150 by assembling both sequences of THC597050 and KIAA1303 in silico, which was confirmed by PCR and d'-rapid amplification of cDNA ends.

UR - https://www.scopus.com/pages/publications/2442679342

M3 - Paper

AN - SCOPUS:2442679342

SP - 751

EP - 752

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

The complete amino acid sequence determination of a novel protein by mass spectrometry with the tentative human consensus sequence database

Abstract

Conference

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