TY - GEN
T1 - Tapak liman (Elephantopus scaber L) extract-induced CD4+ and CD8+ differentiation from hematopoietic stem cells and progenitor cell proliferation in mice (Mus musculus L)
AU - Djati, Muhammad Sasmito
AU - Habibu, Hindun
AU - Jatiatmaja, Nabilah A.
AU - Rifa'I, Muhaimin
N1 - Publisher Copyright:
© 2017 Author(s).
PY - 2017/11/29
Y1 - 2017/11/29
N2 - Tapak Liman (Elephantopus scaber L) is a traditional medicinal plant containing several active compounds that potentially affecting hematopoietic stem cells, such as epifrieelinol, lupeol, stigmasterol, triacontane-1-ol, dotriacontane-1-ol, lupeol acetate, deoxyelephan-topin, isodeoxyelephantopin, polyphenol luteolin-7, as well as various flavonoids and glucosides. The aim of this study was to elucidate the effect of leaf extract of Tapak Liman on hematopoietic stem cells in mice BALB/c, by observation of the relative number of cells expressing CD4/CD8, CD4/CD62L, and TER119/B220 in the spleen, and TER119/B220, TER119/VLA-4 and TER119/CD34 in bone marrow, after being administered leaf extract for 2 weeks. This experiment used 12 female mice, which were divided into three treatment groups, P1= 0.5 g·g bw-1·day-1, P2= 1.0 g·g bw-1·day-1 and P3=2.0 g·g bw-1·day-1 Tapak Liman leaf extract as well as a control. The relative numbers of cells expressing surface molecules were analyzed by flowcytometry and quantitative data were tested using one-way ANOVA. The results showed that the leaf extract of Tapak Liman has no significant effect on erythrocyte proliferation; on the other hand, it had a significant effect on both proliferation and differentiation of B lymphocytes (B220+) in bone marrow (p=0.044) and increased the expression of CD4+, CD8+ molecule in B cells (p=0.026) and erythroid cells in spleen and bone marrow, based on the estimation of cells that expressed TER119+VLA-4+, identified as important in the development pathway of erythrocytes. An increased cell percentage of TER11+VLA-4+ occurred for treatment P2, 12% higher than the control. The increased expression of TER119+VLA-4+ was assumed to be due to the iron content in Tapak Liman, which functioned to stimulate the progenitor hematopoietic cells to proliferate and differentiate into a precursor of erythroid cells (TER119+VLA-4+). There was an increasing number of cells expressing the surface molecules TER119+ and VLA-4+. This indicated that the Tapak Liman leaf extract with a dose of 1.0 g·g bw-1·day-1 could stimulate the proliferation of hematopoietic stem cells into the lymphoid and erythroid pathway, in spleen and bone marrow.
AB - Tapak Liman (Elephantopus scaber L) is a traditional medicinal plant containing several active compounds that potentially affecting hematopoietic stem cells, such as epifrieelinol, lupeol, stigmasterol, triacontane-1-ol, dotriacontane-1-ol, lupeol acetate, deoxyelephan-topin, isodeoxyelephantopin, polyphenol luteolin-7, as well as various flavonoids and glucosides. The aim of this study was to elucidate the effect of leaf extract of Tapak Liman on hematopoietic stem cells in mice BALB/c, by observation of the relative number of cells expressing CD4/CD8, CD4/CD62L, and TER119/B220 in the spleen, and TER119/B220, TER119/VLA-4 and TER119/CD34 in bone marrow, after being administered leaf extract for 2 weeks. This experiment used 12 female mice, which were divided into three treatment groups, P1= 0.5 g·g bw-1·day-1, P2= 1.0 g·g bw-1·day-1 and P3=2.0 g·g bw-1·day-1 Tapak Liman leaf extract as well as a control. The relative numbers of cells expressing surface molecules were analyzed by flowcytometry and quantitative data were tested using one-way ANOVA. The results showed that the leaf extract of Tapak Liman has no significant effect on erythrocyte proliferation; on the other hand, it had a significant effect on both proliferation and differentiation of B lymphocytes (B220+) in bone marrow (p=0.044) and increased the expression of CD4+, CD8+ molecule in B cells (p=0.026) and erythroid cells in spleen and bone marrow, based on the estimation of cells that expressed TER119+VLA-4+, identified as important in the development pathway of erythrocytes. An increased cell percentage of TER11+VLA-4+ occurred for treatment P2, 12% higher than the control. The increased expression of TER119+VLA-4+ was assumed to be due to the iron content in Tapak Liman, which functioned to stimulate the progenitor hematopoietic cells to proliferate and differentiate into a precursor of erythroid cells (TER119+VLA-4+). There was an increasing number of cells expressing the surface molecules TER119+ and VLA-4+. This indicated that the Tapak Liman leaf extract with a dose of 1.0 g·g bw-1·day-1 could stimulate the proliferation of hematopoietic stem cells into the lymphoid and erythroid pathway, in spleen and bone marrow.
UR - https://www.scopus.com/pages/publications/85038908808
U2 - 10.1063/1.5012736
DO - 10.1063/1.5012736
M3 - Conference contribution
AN - SCOPUS:85038908808
T3 - AIP Conference Proceedings
BT - 8th International Conference on Global Resource Conservation, ICGRC 2017
A2 - Kikuchi, Akira
A2 - Salunkhe, Chandrakhant
A2 - Siswanto, Dian
A2 - Jatmiko, Yoga Dwi
A2 - Govinda, Bhupal
PB - American Institute of Physics Inc.
T2 - 8th International Conference on Global Resource Conservation: Green Campus Movement for Global Conservation, ICGRC 2017
Y2 - 19 July 2017 through 20 July 2017
ER -