TY - GEN
T1 - T cell senescence and its correlation to inflammaging process of patients with systemic lupus erythematosus
AU - Ulfah, Fahrina
AU - Octaviani, Hanani
AU - Handono, Kusworini
AU - Kalim, Handono
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/6/4
Y1 - 2019/6/4
N2 - SLE patients suffered from morbidities resembled the natural aging process, suggesting chronic inflammation in SLE is correlated to premature immune senescence. However, understanding of immune senescence in SLE is not well established. The purpose of this study was to investigate the association of CD4+ and CD8+ T cells senescence markers of end differentiated T cell (CD28-), memory T cell (CD45RO+) and cytokines correlated to inflammaging (IL-2, and IFN-γ) among 61 SLE subjects aged 16-56 years. We measured the percentages of T cell senescence by flow cytometric analysis. Serum IL-2 and IFN-γwere measured by ELISA. Among 61 subjects, percentages of SLE subjects with increased senescence cells ranges from 3.2-58.3%. There was a positive correlation between the percentage of senescent T cells and serum IFN-γ(CD4+CD45RO+; p = 0.003, r = 0.453; CD8+CD45RO+; p = 0.045, r = 0.284; and CD4+CD28-; p = 0.029, r = 0.257) and negative correlation for the percentage of senescent T cells and serum IL-2 (CD4+CD28-; p = 0.014, r = -0384; CD8+CD28-; p = 0.012, r = -0394; CD4+CD45RO+; p = 0.023, r = -0.322; and CD8+CD45RO+; p = 0.017, r = -0.335). This study confirms the role of immune senescence in SLE pathogenesis, particularly in regard to the observed loss of CD28 and increased percentages of CD45RO expression from both CD8+ and CD4+ T cells. We also found the inflammaging process in SLE was correlated to T cell senescence. Studies of immune senescence might provide new opportunities for better understanding of SLE pathogenesis.
AB - SLE patients suffered from morbidities resembled the natural aging process, suggesting chronic inflammation in SLE is correlated to premature immune senescence. However, understanding of immune senescence in SLE is not well established. The purpose of this study was to investigate the association of CD4+ and CD8+ T cells senescence markers of end differentiated T cell (CD28-), memory T cell (CD45RO+) and cytokines correlated to inflammaging (IL-2, and IFN-γ) among 61 SLE subjects aged 16-56 years. We measured the percentages of T cell senescence by flow cytometric analysis. Serum IL-2 and IFN-γwere measured by ELISA. Among 61 subjects, percentages of SLE subjects with increased senescence cells ranges from 3.2-58.3%. There was a positive correlation between the percentage of senescent T cells and serum IFN-γ(CD4+CD45RO+; p = 0.003, r = 0.453; CD8+CD45RO+; p = 0.045, r = 0.284; and CD4+CD28-; p = 0.029, r = 0.257) and negative correlation for the percentage of senescent T cells and serum IL-2 (CD4+CD28-; p = 0.014, r = -0384; CD8+CD28-; p = 0.012, r = -0394; CD4+CD45RO+; p = 0.023, r = -0.322; and CD8+CD45RO+; p = 0.017, r = -0.335). This study confirms the role of immune senescence in SLE pathogenesis, particularly in regard to the observed loss of CD28 and increased percentages of CD45RO expression from both CD8+ and CD4+ T cells. We also found the inflammaging process in SLE was correlated to T cell senescence. Studies of immune senescence might provide new opportunities for better understanding of SLE pathogenesis.
KW - immune senescence
KW - Inflammaging
KW - SLE
KW - T cell
UR - https://www.scopus.com/pages/publications/85067061402
U2 - 10.1063/1.5110006
DO - 10.1063/1.5110006
M3 - Conference contribution
AN - SCOPUS:85067061402
T3 - AIP Conference Proceedings
BT - International Conference on Bioinformatics and Nanomedicine from Natural Resources for Biomedical Research
A2 - Budianto, Windy Yuliana
A2 - Sujuti, Hidayat
A2 - Khotimah, Husnul
PB - American Institute of Physics Inc.
T2 - 3rd Annual Scientific Meeting of Indonesian Consortium for Biomedical Sciences, ASMICBS 2018
Y2 - 21 November 2018 through 23 November 2018
ER -