Superdioxide dismutase (SOD) and metallothionein (MT) Tubifex tubifex at the acute mercury exposure

I. M. Widiastuti; A. M.S. Hertika; M. Musa; D. Arfiati

doi:10.1088/1755-1315/236/1/012101

Superdioxide dismutase (SOD) and metallothionein (MT) Tubifex tubifex at the acute mercury exposure

I. M. Widiastuti^*
, A. M.S. Hertika
, M. Musa
, D. Arfiati

^*Corresponding author for this work

Faculty of Fisheries and Marine Sciences

Research output: Contribution to journal › Conference article › peer-review

4 Citations (Scopus)

Abstract

Oxidative stress is a condition of over production of free radicals that can cause cell damage, pathological conditions and cell death. This study aims was to determined the activity of superoxide dismutase (SOD) and metallothionein (MT) levels in Tubifex tubifex worms exposed to HgCl2 in various concentration levels. The concentration of HgCl₂ used was based on LC50-48 h values in acute toxicity test (15,85 μg / L, 25,12 μg / L; 38,81 μg / L; 63,09 μg / L; 99,98 μg / L ). The method used was experiment. Determination of SOD activity done using spectrophotometry and MT measurement using ELISA method. The results was showed that HgCl₂ exposure to Tubifex tubifex caused increased levels of superoxide dismutase enzyme and metallothionein levels. SOD and MT levels were directly proportional to the concentration of HgCl₂, the higher the concentration, SOD and MT levels will increase accordingly.

Original language	English
Article number	012101
Journal	IOP Conference Series: Earth and Environmental Science
Volume	236
Issue number	1
DOIs	https://doi.org/10.1088/1755-1315/236/1/012101
Publication status	Published - 1 Mar 2019
Event	1st International Conference on Fisheries and Marine Science, InCoFiMS 2018 - East Java, Indonesia Duration: 6 Oct 2018 → …

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SDG 3 Good Health and Well-being

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10.1088/1755-1315/236/1/012101

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title = "Superdioxide dismutase (SOD) and metallothionein (MT) Tubifex tubifex at the acute mercury exposure",

abstract = "Oxidative stress is a condition of over production of free radicals that can cause cell damage, pathological conditions and cell death. This study aims was to determined the activity of superoxide dismutase (SOD) and metallothionein (MT) levels in Tubifex tubifex worms exposed to HgCl2 in various concentration levels. The concentration of HgCl2 used was based on LC50-48 h values in acute toxicity test (15,85 μg / L, 25,12 μg / L; 38,81 μg / L; 63,09 μg / L; 99,98 μg / L ). The method used was experiment. Determination of SOD activity done using spectrophotometry and MT measurement using ELISA method. The results was showed that HgCl2 exposure to Tubifex tubifex caused increased levels of superoxide dismutase enzyme and metallothionein levels. SOD and MT levels were directly proportional to the concentration of HgCl2, the higher the concentration, SOD and MT levels will increase accordingly.",

author = "Widiastuti, \{I. M.\} and Hertika, \{A. M.S.\} and M. Musa and D. Arfiati",

note = "Publisher Copyright: {\textcopyright} Published under licence by IOP Publishing Ltd.; 1st International Conference on Fisheries and Marine Science, InCoFiMS 2018 ; Conference date: 06-10-2018",

year = "2019",

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doi = "10.1088/1755-1315/236/1/012101",

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TY - JOUR

T1 - Superdioxide dismutase (SOD) and metallothionein (MT) Tubifex tubifex at the acute mercury exposure

AU - Widiastuti, I. M.

AU - Hertika, A. M.S.

AU - Musa, M.

AU - Arfiati, D.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Oxidative stress is a condition of over production of free radicals that can cause cell damage, pathological conditions and cell death. This study aims was to determined the activity of superoxide dismutase (SOD) and metallothionein (MT) levels in Tubifex tubifex worms exposed to HgCl2 in various concentration levels. The concentration of HgCl2 used was based on LC50-48 h values in acute toxicity test (15,85 μg / L, 25,12 μg / L; 38,81 μg / L; 63,09 μg / L; 99,98 μg / L ). The method used was experiment. Determination of SOD activity done using spectrophotometry and MT measurement using ELISA method. The results was showed that HgCl2 exposure to Tubifex tubifex caused increased levels of superoxide dismutase enzyme and metallothionein levels. SOD and MT levels were directly proportional to the concentration of HgCl2, the higher the concentration, SOD and MT levels will increase accordingly.

AB - Oxidative stress is a condition of over production of free radicals that can cause cell damage, pathological conditions and cell death. This study aims was to determined the activity of superoxide dismutase (SOD) and metallothionein (MT) levels in Tubifex tubifex worms exposed to HgCl2 in various concentration levels. The concentration of HgCl2 used was based on LC50-48 h values in acute toxicity test (15,85 μg / L, 25,12 μg / L; 38,81 μg / L; 63,09 μg / L; 99,98 μg / L ). The method used was experiment. Determination of SOD activity done using spectrophotometry and MT measurement using ELISA method. The results was showed that HgCl2 exposure to Tubifex tubifex caused increased levels of superoxide dismutase enzyme and metallothionein levels. SOD and MT levels were directly proportional to the concentration of HgCl2, the higher the concentration, SOD and MT levels will increase accordingly.

UR - https://www.scopus.com/pages/publications/85063373224

U2 - 10.1088/1755-1315/236/1/012101

DO - 10.1088/1755-1315/236/1/012101

M3 - Conference article

AN - SCOPUS:85063373224

SN - 1755-1307

VL - 236

JO - IOP Conference Series: Earth and Environmental Science

JF - IOP Conference Series: Earth and Environmental Science

IS - 1

M1 - 012101

T2 - 1st International Conference on Fisheries and Marine Science, InCoFiMS 2018

Y2 - 6 October 2018

ER -

Superdioxide dismutase (SOD) and metallothionein (MT) Tubifex tubifex at the acute mercury exposure

Abstract

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