Study of various solvents for caffeine determination using UV spectrophotometeric

Caffeine acts as a stimulant drug. In humans, caffeine acts as a central nervous system stimulant, temporarily warding off drowsiness and restoring alertness. It is the world's most widely consumed psychoactive drug, but, unlike many other psychoactive substances, it is both legal and unregulated in nearly all parts of the world. Because of the wide spread consumption of caffeine, it is important to collect precise information of their content in types of drinks. Most research activities have been focused on chromatographic such as HPLC methods; however, spectra-photometric determination is preferred because of its rapidity, high accuracy, simplicity and reproducibility. Advantages of UV Specto photometric is cheap and it is found in many laboratories. But caffeine content in tea leaves cannot be determined directly using UV visible spectrometer, due to the matrix effect of UV absorbing substances. Simple and accurate method for the determination of Caffeine content in tea leaves directly using UV-visible spectrometer was developed. Standard linear calibration curve with accepted correlation value was obtained.