Splenectomy and salmonella typhi exposure lead to quantitative alterations of bone marrow B220⁺ cells and Cd4⁺ T cells on BALB/c mice

Objectives: This study aims to determine the quantitative alterations of relative density of bone marrow B220⁺ cells a nd CD4⁺ T cells in BALB/c mice which underwent splenectomy and exposed to Salmonella typhi (S. typhi). Materials and methods: Splenectomy was performed under ketamine anesthesia (65 mg/kg BW). Exposure of S. typhi was performed by injection of acute doses (109 c ells/mL) a t t wo w eeks a fter s plenectomy a nd repeated at two weeks after the initial dose. The relative density of bone marrow B220⁺ cells and CD4⁺ T cells were quantified by BD FACSCalibur™ f low c ytometer. S tatistical a nalysis w as p erformed u sing t wo-way ANOVA and Tukey HSD test. A p value of <0.05 was considered significant. Results: All treatments had significant effects on observed variables. Splenectomy significantly depleted the relative density of B220⁺ cells to 5.51% compare with controls (17.34%). Salmonella typhi exposure alone did not significantly alter the relative density of B220⁺ cells (17.92%). Splenectomy and S. typhi exposure decreased B220⁺ cells to 5.90%. CD4⁺ T cells had various responses to all treatments. Compared to the controls (0.28%), splenectomy significantly increased the relative density of CD4⁺ T cells up to 0.43%. Salmonella typhi exposure alone and splenectomy in combined with S. typhi exposure significantly increased the relative density of CD4⁺ T cells up to 0.55% and 0.50%, respectively. Conclusion: These results indicated that splenectomy was the leading factor affecting the relative density of B220⁺ cells, rather than S. typhi exposure, whereas Salmonella typhi exposure was the main factor which increased the relative density of CD4⁺ T cells. This phenomenon also reflects the pathological condition due to S. typhi exposure.