Significance of EMSA eritin administration on erythropoiesis and complement regulators in irradiated mice

Objectives: This study aims to examine the effect of polyherbal erythropoiesis modulatory and stimulatory agent (EMSA) Eritin administration in modulating the activity of erythropoiesis on TER-119⁺VLA-4⁺ cells and complement regulators TER-119⁺CD55⁺ a nd T ER-119⁺CD59⁺ cells in Balb/c mice after total body irradiation (TBI) with a dose of 500 rad. Materials and methods: EMSA Eritin was administered orally in mice after exposure to TBI with a dose of 500 rad daily for two weeks with three different doses including a low dose (1.04 mg/g BW), normal dose (3.125 mg/g BW), and high dose (9.375 mg/g BW). Mice were treated with HEMAPO Epoetin alfa twice a week as positive control with a dose of 0.21 mg/g BW. On day 15, the bone marrow was isolated and the number of TER-119⁺VLA-4⁺, TER-119⁺CD55⁺ a nd T ER-119⁺CD59⁺ cells were a nalyzed using flow cytometry. Examination showed marked reduction in the number of TER-119⁺VLA-4⁺ cells in the bone marrow of mice after TBI with dose of 500 rad compared to those of healthy controls. The number of TER-119⁺VLA-4⁺ cells in the irradiated m ice was markedly increased with EMSA Eritin from low dose to high dose compared to mice with TBI and mice treated with Epoetin alfa. Results: The highest dose of EMSA Eritin was more effective to promote erythropoiesis than HEMAPO Epoetin alfa and the other dose of EMSA Eritin. Conclusion: Our study findings suggest that EMSA Eritin is a powerful medicinal herbal supplement which may serve as a protective agent to normalize homeostasis and erythropoiesis after irradiation with a dose of 500 rad.