SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia

Mardiana Lelitawati; Kuntaman Kuntaman; Eko Budi Koendhori; Dewi Santosaningsih; Aima Insana

doi:10.1063/5.0234716

SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia

Mardiana Lelitawati^*
, Kuntaman Kuntaman
, Eko Budi Koendhori
, Dewi Santosaningsih
, Aima Insana

^*Corresponding author for this work

Faculty of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

MRSA strains can grow and spread quickly due to the ability to obtain and utilize mobile genetic elements such as SCCmec. Identification of SCCmec type in health care centers is essential to know the likely source of the spread of MRSA. This study aimed to analyze the distribution of SCCmec type in isolates of MRSA taken from surgical ward patients in Dr. Soetomo Hospital, Surabaya. The samples were 30 isolates from previous studies identified as MRSA colonization in the nose and throat. Isolates were inoculated onto Mannitol Salt Agar (MSA) and then subcultured onto Brilliance MRSA 2 Agar, followed by catalase and coagulase test. The PCR method has amplified thirty DNA templates to detect the SCCmec type. The result showed that 30/30 isolates (100%) contained SCCmec type III (HA-MRSA). Most of the admission patients in surgical wards were referral patients from the secondary hospital, so patients possibly entered the wards already with HA-MRSA colonization. Discharge patients were mostly hospitalized for a long time, with the duration of hospitalization being 6 to 132 days, with an average of 35.4 days. Hospitalizations allow the spread of MRSA from patients infected or colonized with MRSA SCCmec type III to healthcare workers and vice versa.

Original language	English
Title of host publication	AIP Conference Proceedings
Editors	Ahmad Taufiq, Hendra Susanto, Chuang-Rung Chang, I. Ching Wang, Hadi Nur, Muhammad Aziz, Sri Rahayu Lestari, Markus Diantoro, Nandang Mufti, Nik Ahmad Nizam Nik Malek, Sunaryono, Siti Zubaidah, Aulanni'am, Indra Wibowo, Adeodatus Yuda Handaya
Publisher	American Institute of Physics
Edition	1
ISBN (Electronic)	9780735451025
DOIs	https://doi.org/10.1063/5.0234716
Publication status	Published - 30 Jan 2025
Event	5th International Conference on Life Science and Technology, ICoLiST 2022 - Hybrid, Malang, Indonesia Duration: 30 Aug 2022 → 31 Aug 2022

Publication series

Name	AIP Conference Proceedings
Number	1
Volume	3186
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	5th International Conference on Life Science and Technology, ICoLiST 2022
Country/Territory	Indonesia
City	Hybrid, Malang
Period	30/08/22 → 31/08/22

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1063/5.0234716

Cite this

Lelitawati, M., Kuntaman, K., Koendhori, E. B., Santosaningsih, D., & Insana, A. (2025). SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia. In A. Taufiq, H. Susanto, C.-R. Chang, I. C. Wang, H. Nur, M. Aziz, S. R. Lestari, M. Diantoro, N. Mufti, N. A. N. N. Malek, Sunaryono, S. Zubaidah, Aulanni'am, I. Wibowo, & A. Y. Handaya (Eds.), AIP Conference Proceedings (1 ed.). Article 020054 (AIP Conference Proceedings; Vol. 3186, No. 1). American Institute of Physics. https://doi.org/10.1063/5.0234716

Lelitawati, Mardiana ; Kuntaman, Kuntaman ; Koendhori, Eko Budi et al. / SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia. AIP Conference Proceedings. editor / Ahmad Taufiq ; Hendra Susanto ; Chuang-Rung Chang ; I. Ching Wang ; Hadi Nur ; Muhammad Aziz ; Sri Rahayu Lestari ; Markus Diantoro ; Nandang Mufti ; Nik Ahmad Nizam Nik Malek ; Sunaryono ; Siti Zubaidah ; Aulanni'am ; Indra Wibowo ; Adeodatus Yuda Handaya. 1. ed. American Institute of Physics, 2025. (AIP Conference Proceedings; 1).

@inproceedings{7bca7942de7647bc8e46f11e7644046b,

title = "SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia",

abstract = "MRSA strains can grow and spread quickly due to the ability to obtain and utilize mobile genetic elements such as SCCmec. Identification of SCCmec type in health care centers is essential to know the likely source of the spread of MRSA. This study aimed to analyze the distribution of SCCmec type in isolates of MRSA taken from surgical ward patients in Dr. Soetomo Hospital, Surabaya. The samples were 30 isolates from previous studies identified as MRSA colonization in the nose and throat. Isolates were inoculated onto Mannitol Salt Agar (MSA) and then subcultured onto Brilliance MRSA 2 Agar, followed by catalase and coagulase test. The PCR method has amplified thirty DNA templates to detect the SCCmec type. The result showed that 30/30 isolates (100\%) contained SCCmec type III (HA-MRSA). Most of the admission patients in surgical wards were referral patients from the secondary hospital, so patients possibly entered the wards already with HA-MRSA colonization. Discharge patients were mostly hospitalized for a long time, with the duration of hospitalization being 6 to 132 days, with an average of 35.4 days. Hospitalizations allow the spread of MRSA from patients infected or colonized with MRSA SCCmec type III to healthcare workers and vice versa.",

author = "Mardiana Lelitawati and Kuntaman Kuntaman and Koendhori, \{Eko Budi\} and Dewi Santosaningsih and Aima Insana",

note = "Publisher Copyright: {\textcopyright} 2025 American Institute of Physics Inc.. All rights reserved.; 5th International Conference on Life Science and Technology, ICoLiST 2022 ; Conference date: 30-08-2022 Through 31-08-2022",

year = "2025",

month = jan,

day = "30",

doi = "10.1063/5.0234716",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics",

number = "1",

editor = "Ahmad Taufiq and Hendra Susanto and Chuang-Rung Chang and Wang, \{I. Ching\} and Hadi Nur and Muhammad Aziz and Lestari, \{Sri Rahayu\} and Markus Diantoro and Nandang Mufti and Malek, \{Nik Ahmad Nizam Nik\} and Sunaryono and Siti Zubaidah and Aulanni'am and Indra Wibowo and Handaya, \{Adeodatus Yuda\}",

booktitle = "AIP Conference Proceedings",

edition = "1",

}

Lelitawati, M, Kuntaman, K, Koendhori, EB, Santosaningsih, D & Insana, A 2025, SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia. in A Taufiq, H Susanto, C-R Chang, IC Wang, H Nur, M Aziz, SR Lestari, M Diantoro, N Mufti, NANN Malek, Sunaryono, S Zubaidah, Aulanni'am, I Wibowo & AY Handaya (eds), AIP Conference Proceedings. 1 edn, 020054, AIP Conference Proceedings, no. 1, vol. 3186, American Institute of Physics, 5th International Conference on Life Science and Technology, ICoLiST 2022, Hybrid, Malang, Indonesia, 30/08/22. https://doi.org/10.1063/5.0234716

SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia. / Lelitawati, Mardiana; Kuntaman, Kuntaman; Koendhori, Eko Budi et al.
AIP Conference Proceedings. ed. / Ahmad Taufiq; Hendra Susanto; Chuang-Rung Chang; I. Ching Wang; Hadi Nur; Muhammad Aziz; Sri Rahayu Lestari; Markus Diantoro; Nandang Mufti; Nik Ahmad Nizam Nik Malek; Sunaryono; Siti Zubaidah; Aulanni'am; Indra Wibowo; Adeodatus Yuda Handaya. 1. ed. American Institute of Physics, 2025. 020054 (AIP Conference Proceedings; Vol. 3186, No. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia

AU - Lelitawati, Mardiana

AU - Kuntaman, Kuntaman

AU - Koendhori, Eko Budi

AU - Santosaningsih, Dewi

AU - Insana, Aima

PY - 2025/1/30

Y1 - 2025/1/30

N2 - MRSA strains can grow and spread quickly due to the ability to obtain and utilize mobile genetic elements such as SCCmec. Identification of SCCmec type in health care centers is essential to know the likely source of the spread of MRSA. This study aimed to analyze the distribution of SCCmec type in isolates of MRSA taken from surgical ward patients in Dr. Soetomo Hospital, Surabaya. The samples were 30 isolates from previous studies identified as MRSA colonization in the nose and throat. Isolates were inoculated onto Mannitol Salt Agar (MSA) and then subcultured onto Brilliance MRSA 2 Agar, followed by catalase and coagulase test. The PCR method has amplified thirty DNA templates to detect the SCCmec type. The result showed that 30/30 isolates (100%) contained SCCmec type III (HA-MRSA). Most of the admission patients in surgical wards were referral patients from the secondary hospital, so patients possibly entered the wards already with HA-MRSA colonization. Discharge patients were mostly hospitalized for a long time, with the duration of hospitalization being 6 to 132 days, with an average of 35.4 days. Hospitalizations allow the spread of MRSA from patients infected or colonized with MRSA SCCmec type III to healthcare workers and vice versa.

AB - MRSA strains can grow and spread quickly due to the ability to obtain and utilize mobile genetic elements such as SCCmec. Identification of SCCmec type in health care centers is essential to know the likely source of the spread of MRSA. This study aimed to analyze the distribution of SCCmec type in isolates of MRSA taken from surgical ward patients in Dr. Soetomo Hospital, Surabaya. The samples were 30 isolates from previous studies identified as MRSA colonization in the nose and throat. Isolates were inoculated onto Mannitol Salt Agar (MSA) and then subcultured onto Brilliance MRSA 2 Agar, followed by catalase and coagulase test. The PCR method has amplified thirty DNA templates to detect the SCCmec type. The result showed that 30/30 isolates (100%) contained SCCmec type III (HA-MRSA). Most of the admission patients in surgical wards were referral patients from the secondary hospital, so patients possibly entered the wards already with HA-MRSA colonization. Discharge patients were mostly hospitalized for a long time, with the duration of hospitalization being 6 to 132 days, with an average of 35.4 days. Hospitalizations allow the spread of MRSA from patients infected or colonized with MRSA SCCmec type III to healthcare workers and vice versa.

UR - https://www.scopus.com/pages/publications/85217704656

U2 - 10.1063/5.0234716

DO - 10.1063/5.0234716

M3 - Conference contribution

AN - SCOPUS:85217704656

T3 - AIP Conference Proceedings

BT - AIP Conference Proceedings

A2 - Taufiq, Ahmad

A2 - Susanto, Hendra

A2 - Chang, Chuang-Rung

A2 - Wang, I. Ching

A2 - Nur, Hadi

A2 - Aziz, Muhammad

A2 - Lestari, Sri Rahayu

A2 - Diantoro, Markus

A2 - Mufti, Nandang

A2 - Malek, Nik Ahmad Nizam Nik

A2 - Sunaryono, null

A2 - Zubaidah, Siti

A2 - Aulanni'am, null

A2 - Wibowo, Indra

A2 - Handaya, Adeodatus Yuda

PB - American Institute of Physics

T2 - 5th International Conference on Life Science and Technology, ICoLiST 2022

Y2 - 30 August 2022 through 31 August 2022

ER -

Lelitawati M, Kuntaman K, Koendhori EB, Santosaningsih D, Insana A. SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia. In Taufiq A, Susanto H, Chang CR, Wang IC, Nur H, Aziz M, Lestari SR, Diantoro M, Mufti N, Malek NANN, Sunaryono, Zubaidah S, Aulanni'am, Wibowo I, Handaya AY, editors, AIP Conference Proceedings. 1 ed. American Institute of Physics. 2025. 020054. (AIP Conference Proceedings; 1). doi: 10.1063/5.0234716

SCCmec Typing in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Surgical Wards Patients, Dr. Soetomo Hospital, Surabaya, Indonesia

Abstract

Publication series

Conference

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this