Rhodococcus pyridinivorans TPIK-Derived Silver Nanoparticles for Colorimetric Cyanide Detection in Food

Cyanide is a toxic compound and often found in broad-ranging of foods in the form of cyanogenic glycosides. Consuming foods with high cyanide concentrations could lead to cyanide poisoning, which may cause symptoms such as hyperventilation, seizure, loss of consciousness, or even death. However, despite the signiRicant health risks, accesible and user-friendly methods for cyanide detection are still limited. In the present work, we developed a colorimetric paper-based analytical device (PAD) for detecting cyanide in food. The device was made by incorporating biogenic silver nanoparticles (AgNPs) into Whatman Rilter paper, with the AgNPs being synthesized using a cell extract from Rhodococcus pyridinivorans TPIK. The PAD demonstrated high sensitivity to cyanide ions, with a limit of detection (LOD) of 54,95 ppm and a limit of quantiRication (LOQ) 166,53 ppm. It also exhibited excellent selectivity for cyanide over common anions, including Ca²⁺, K⁺, Na⁺, SO4^2-, and Cl- and remained stable at room temperature for up to 14 days.