@inproceedings{4bfd563f820c47a592a2d5fca75eca0e,
title = "Revealing Spleen Ad4BP/SF1 Knockout Mouse by BAC-Ad4BP-tTAZ Transgene",
abstract = "The purpose of this study is addressed the function of Ad4BP/SF1 in developing spleen of Ad4BP/SF-1 KO mouse. I have modified the BAC-Ad4BP endogenous by homologous recombinant with modification cassette-containing tetracycline transactivator system and LacZ reporter genes to allow the expression of Ad4BP/SF1 in developing tissues and organ differentiation, regulate of Ad4BP/SF1 mechanism, and reveal the defect due to Ad4BP/SF1 deficiency. Recently studies, by immunihistochemistry of adult tissues showed that revealed coincident expressing between Ad4BP/SF-1 and lacZ, indicating the tTA system seemed to reproduces the endogenous expression of Ad4BP/SF-1, and thus likely to lacZ, Ad4BP/SF-1 in the BAC transgene was expected to be expressed in the corresponding tissues under the control of the bidirectional promoter. Interestingly, the BAC-Ad4BP-tTA transgenic mice were succeeded to up regulate the expression of Ad4BP/SF-1 at the protein level than wild type. And the fetal spleen of Tg(+);Ad4(+/+) was larger than that of the wild type, and expectedly the size of the spleen of Tg(+);Ad4(-/-) apparently recovered.",
keywords = "Ad4BP/SF-1, BAC transgenic, homologous recombinant, knockout mouse, Spleen",
author = "Fatchiyah and M. Zubair and Morohashi, \{K. I.\}",
year = "2009",
doi = "10.1007/978-3-540-92841-6\_477",
language = "English",
isbn = "9783540928409",
series = "IFMBE Proceedings",
pages = "1920--1923",
booktitle = "13th International Conference on Biomedical Engineering - ICBME 2008",
note = "13th International Conference on Biomedical Engineering, ICBME 2008 ; Conference date: 03-12-2008 Through 06-12-2008",
}