Retinal digest procedures for examining pericyte and endothelial on retinal vasculature using rat model

Retinal digest procedures is a gold standard method for examine anatomical details of retinal vasculature includes pericyte, endothelial cells, and basement membrane. Knowing the details of retinal vasculature helps us to study and understand more about pathological changes of many retinal vascular disorder, for example diabetic retinopathy. The aimedof this study was to make a modification methodsfrom previously literature. This study using rat model, also give some trick to avoid the fail of making retinal digest in order to make guidance for the next experimental study. The result showed that the pericyte and endothelial cells in retinal vasculature can be seen clearly using this methods.