TY - GEN
T1 - Removal of humic acid from peat soils by using AlCl3 prior to DNA extraction
AU - Mustafa, Irfan
AU - Hadiatullah,
AU - Sustiyah,
N1 - Publisher Copyright:
© 2017 Author(s).
PY - 2017/5/17
Y1 - 2017/5/17
N2 - The amplification of environmental DNA is one of the main steps in microbial diversity profiling of environmental samples. To represent the microbial community in the soil, DNA extraction is initially needed. The major inhibitor in the soil is a humic acid which greatly inhibits the ability of enzymes to amplify DNA. The protocol provided with the commercial kit was not able to resolve the problem. We, therefore, introduced a modification for soil sample treatment with AlCl3 as a flocculating agent that is capable of removing the humic substance contained in peat soil. This technique was superior to the original instruction for extracting DNA with the FastDNA® Spin Kit for Soil (MP Bio, USA).
AB - The amplification of environmental DNA is one of the main steps in microbial diversity profiling of environmental samples. To represent the microbial community in the soil, DNA extraction is initially needed. The major inhibitor in the soil is a humic acid which greatly inhibits the ability of enzymes to amplify DNA. The protocol provided with the commercial kit was not able to resolve the problem. We, therefore, introduced a modification for soil sample treatment with AlCl3 as a flocculating agent that is capable of removing the humic substance contained in peat soil. This technique was superior to the original instruction for extracting DNA with the FastDNA® Spin Kit for Soil (MP Bio, USA).
UR - https://www.scopus.com/pages/publications/85020215213
U2 - 10.1063/1.4983434
DO - 10.1063/1.4983434
M3 - Conference contribution
AN - SCOPUS:85020215213
T3 - AIP Conference Proceedings
BT - 7th International Conference on Global Resource Conservation
A2 - Mustafa, Irfan
A2 - Shrestha, Bhupal Govinda
A2 - Hamid, Nazefah Abdul
PB - American Institute of Physics Inc.
T2 - 7th International Conference on Global Resource Conservation: From Traditional Herbal Medicine into Synthetic Biology for Better Human Lives, ICGRC 2016
Y2 - 2 November 2016 through 4 November 2016
ER -