Quality and Proportion of X and Y Sperm After Sexing Process using Percoll Density Gradient Centrifugation Method on Different Gradients and Diluents in Belgian Blue Cross Bull

This study aimed to evaluate the success rate of the sexing method using Percoll density gradient centrifugation on various gradients and diluents in Belgian Blue bull. The research material used was a semen sample of Belgian Blue cross bull with progressive motility of fresh semen of≥ 70%. The research used a Completely Randomized Design (CRD) with four treatments and ten replications. Data were analyzed using the Tukey test. The treatment in the study was T0: sexing PDGC 10 fractions using Andromed® diluent. T1: sexing PDGC 10 fractions using tris aminomethane diluent + 20% egg yolk. T2: sexing PDGC 5 fractions using Andromed® diluent. T43: sexing PDGC 10 fractions using tris aminomethane diluent + 20% egg yolk. Parameters measured include motility, viability, abnormalities, concentration, Total motile sperm, and proportion of sperm. The results of the analysis of variance showed significant differences in the upper layer for motility, concentration, and Total motile sperm (P<0.01), as well as in the lower layer for Motility and Total motile sperm (P<0.05). There were no significant differences in concentrations between treatments. Viability and abnormality showed significant differences in both layers (P<0.01). The proportion of sperm produced met the expected value of 70%. In conclusion, Andromed® diluent and tris aminomethane + 20% egg yolk can maintain the quality and proportion of sperm, and further analysis needs to be carried out regarding the use of Andromed® diluent and tris aminomethane + 20% egg yolk in the sexing semen freezing process.