TY - GEN
T1 - Propolis ethanolic extract influenced biofilm formation and gene expression level of EFG1 in Candida albicans
AU - Prawiro, Sumarno Reto
AU - Dewi, A. A.Santi
AU - Diniayuningrum, Anggie
AU - Maghfiroh, Dinda Oktia
AU - Nurdiana, Nurdiana
AU - Endharti, Agustina Tri
AU - Rahardjo, Bambang
N1 - Publisher Copyright:
© 2021 Author(s).
PY - 2021/5/25
Y1 - 2021/5/25
N2 - Vulvovaginal candidiasis occurs in 70-75% of women. About 40-45% of them experience vulvovaginal candidiasis for the second time. Approximately 70-89% of these infections are caused by Candida albicans. Candida albicans forming biofilm are reported to have a higher resistance to most anti-fungal drugs, including fluconazole. Objective: To determine the effects of propolis ethanolic extract (PEE) on biofilm formation and genes expression level of EFG1 on C. albicans biofilm. Methods: C. albicans biofilm formation was grown on SDB + 2% glucose and added with PEE with a concentration of 0% (control group) and 2.5, 5, 7.5, 10 and 12.5% (treatment group). Then, C. albicans biofilm formation was incubated at 37 °C for 48 hours. Biofilm inhibition was tested by the crystal violet assay. Analysis of genes expression level of EFG1 was performed using Real Time-PCR and calculated by absolute quantification method. Results: The results of the PEE effect on biofilm inhibition percentage showed a decrease in biofilm formation with the increase in PEE concentration given (p = 0.001), and gene expression level analysis showed that EFG1 (p = 0.000) expression level decreased with the increase in PEE concentration given. Conclusion: Propolis ethanolic extract could reduce biofilm formation and down-regulate the expression of EFG1 in C. albicans biofilm formation.
AB - Vulvovaginal candidiasis occurs in 70-75% of women. About 40-45% of them experience vulvovaginal candidiasis for the second time. Approximately 70-89% of these infections are caused by Candida albicans. Candida albicans forming biofilm are reported to have a higher resistance to most anti-fungal drugs, including fluconazole. Objective: To determine the effects of propolis ethanolic extract (PEE) on biofilm formation and genes expression level of EFG1 on C. albicans biofilm. Methods: C. albicans biofilm formation was grown on SDB + 2% glucose and added with PEE with a concentration of 0% (control group) and 2.5, 5, 7.5, 10 and 12.5% (treatment group). Then, C. albicans biofilm formation was incubated at 37 °C for 48 hours. Biofilm inhibition was tested by the crystal violet assay. Analysis of genes expression level of EFG1 was performed using Real Time-PCR and calculated by absolute quantification method. Results: The results of the PEE effect on biofilm inhibition percentage showed a decrease in biofilm formation with the increase in PEE concentration given (p = 0.001), and gene expression level analysis showed that EFG1 (p = 0.000) expression level decreased with the increase in PEE concentration given. Conclusion: Propolis ethanolic extract could reduce biofilm formation and down-regulate the expression of EFG1 in C. albicans biofilm formation.
UR - https://www.scopus.com/pages/publications/85107265023
U2 - 10.1063/5.0052928
DO - 10.1063/5.0052928
M3 - Conference contribution
AN - SCOPUS:85107265023
T3 - AIP Conference Proceedings
BT - International Conference on Life Sciences and Technology, ICoLiST 2020
A2 - Taufiq, Ahmad
A2 - Susanto, Hendra
A2 - Nur, Hadi
A2 - Aziz, Muhammad
A2 - Suksuwan, Acharee
A2 - Ng, Chen Siang
A2 - Jemon, Khairunadwa Binti
A2 - Amin, Mohamad
A2 - Diantoro, Markus
A2 - Mufti, Nandang
A2 - Malek, Nik Ahmad Nizam Nik
A2 - Wang, I. Ching
A2 - Sunaryono, null
A2 - Zubaidah, Siti
A2 - Aulanni'am, null
A2 - Wibowo, Indra
A2 - Handaya, Adeodatus Yuda
PB - American Institute of Physics Inc.
T2 - 3rd International Conference on Life Sciences and Technology, ICoLiST 2020
Y2 - 29 September 2020
ER -