TY - GEN
T1 - Proliferation and viability of preosteoblast cells treated with Katsuwonus pelamis bone hydroxyapatite
AU - Wardani, Sinta Candra
AU - Sujuti, Hidayat
AU - Mustamsir, Edi
AU - Hapsari, Diwya Nugrahini
N1 - Publisher Copyright:
© 2022 Author(s).
PY - 2022/11/16
Y1 - 2022/11/16
N2 - Hydroxyapatite is an alloplast material that is quite good as a bone graft candidate for bone regeneration. There are many studies examining the production of hydroxyapatite from various natural materials. In this study, used hydroxyapatite produced from Katsuwonus pelamis bones. The HA produced by the precipitation method produced white hydroxyapatite powder which was then further tested for its biological properties against preosteoblast cell cultures. Cell proliferation assay was performed using MTT assay. The treatment group were treated with 3 different concentration. The treatment group were treated with 3 different concentrations; 200, 100 and 50 μg/ml and incubated for 24, 48 and 72 hours. Cell viability assay was performed using flowcytometry. The treatment group were added HA with a concentration of 50 μg/ml then incubated for 72 hours and then stained with Annexin V-PI kit. The results of the viability test show that hydroxyapatite is biocompatible and non-toxic. However, it has not been sufficient to increase the proliferation of preosteoblast cells.
AB - Hydroxyapatite is an alloplast material that is quite good as a bone graft candidate for bone regeneration. There are many studies examining the production of hydroxyapatite from various natural materials. In this study, used hydroxyapatite produced from Katsuwonus pelamis bones. The HA produced by the precipitation method produced white hydroxyapatite powder which was then further tested for its biological properties against preosteoblast cell cultures. Cell proliferation assay was performed using MTT assay. The treatment group were treated with 3 different concentration. The treatment group were treated with 3 different concentrations; 200, 100 and 50 μg/ml and incubated for 24, 48 and 72 hours. Cell viability assay was performed using flowcytometry. The treatment group were added HA with a concentration of 50 μg/ml then incubated for 72 hours and then stained with Annexin V-PI kit. The results of the viability test show that hydroxyapatite is biocompatible and non-toxic. However, it has not been sufficient to increase the proliferation of preosteoblast cells.
UR - https://www.scopus.com/pages/publications/85142896189
U2 - 10.1063/5.0098998
DO - 10.1063/5.0098998
M3 - Conference contribution
AN - SCOPUS:85142896189
T3 - AIP Conference Proceedings
BT - 3rd International Seminar on Smart Molecule of Natural Resources-Asian Federation Biotechnology, ISSMART-AFOB 2021
A2 - Fatchiyah, Fatchiyah
A2 - Sari, Dewi Ratih Tirto
A2 - Kurnianingsih, Nia
A2 - Safitri, Anna
PB - American Institute of Physics Inc.
T2 - 3rd International Seminar on Smart Molecule of Natural Resources-Asian Federation Biotechnology, ISSMART-AFOB 2021
Y2 - 25 August 2021 through 26 August 2021
ER -