Printed low-cost microfluidic paper-based analytical devices for quantitative detection of vitamin c in fruits

Microfluidic paper-based analytical device has been fabricated and subsequently applied to determine the Vitamin C level of the non-seasonal fruit i.e pineapples, oranges, guavas, and apples in Indonesia. PAD was prepared by creating a hydrophobic barrier on Whatman Chromatography paper No. 1 in a certain pattern printed using Xerox Colorcube 8580 DN-2. 0.05 l of 2,6-Dichlorophenolindophenol was dispersed into a detection area on the PAD devices prior to the addition of 0.2 l of fruits juice samples. The barrier allowed the fruits juice to flow and alter the color of the reagents through a redox reaction. Vitamin C in fruit juice altered the color of 2.6-Dichlorophenolindophenol reagent into a pinkish color. The changing of the reagent color was then processed using Image J software to determine the RGB intensity level. The results showed that the average amounts of Vitamin C in pineapples, oranges, guavas, and apples are 0.05%, 0.05%, 0.08%, and 0.04% respectively.