@inproceedings{b987e6d398c0452a9cc0920933388737,
title = "Plantlet regeneration and acclimatization from gamma-irradiated in vitro shoots of vetiver (Vetiveria zizanioides (L.) nash)",
abstract = "The vetiver oil production in Indonesia decreases yearly due to its low quality, so it is necessary to develop superior vetiver plants through in vitro mutagenesis. This research aimed to evaluate the gamma-ray irradiation effect on vetiver plantlet regeneration and acclimatization. The in vitro shoots were irradiated with gamma-rays at doses of 0, 15, 30, 45, 60, and 75 Gy. Irradiated shoots were multiplied and regenerated into plantlets on culture medium, then acclimatized on the soil growing medium. The results showed that gamma-ray irradiation on shoots culture inhibited plantlet regeneration and acclimatization but did not significantly affect plantlet height growth. Inhibition in the plantlet regeneration capacity of irradiated shoots occurred at high irradiation doses (45-75 Gy). The plantlet regeneration capacity of non-irradiated and irradiated shoots at low doses (15-30 Gy) was 100\%, while at high doses (45-75 Gy), it was 72-92\%; regeneration capacity decreased with increasing irradiation doses. Plantlets regenerated from non-irradiated and irradiated shoots had good acclimatization survival (100\%). The survival frequency decreased (80\%) at the acclimatization stage only occurred at a high dose of 75 Gy.",
keywords = "irradiated, mutagenesis, plantlet, regeneration, Vetiver",
author = "M. Rohmah and S. Indriyani and W. Widoretno",
note = "Publisher Copyright: {\textcopyright} 2023 Author(s).; 2nd Sriwijaya International Conference of Basic and Applied Sciences 2021, SICBAS 2021 ; Conference date: 02-11-2021",
year = "2023",
month = dec,
day = "28",
doi = "10.1063/5.0179720",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
number = "1",
editor = "Minetaka Sugiyama and Orlandi, \{Marcelo Ornaghi\} and Arief Gusnanto and Walid Fouad and Majid, \{Fadzilah Adibah Abdul\} and Hariani, \{Poedji Loekitowati\} and Muhammad Said and Robbi Rahim",
booktitle = "AIP Conference Proceedings",
edition = "1",
}