Phytochemical screening, LC-MS/MS, and antibacterial evaluation from the extract of leaves Pinus merkusii Jung et de Vriese

Brawijaya University Forest occupies an area of about 514 hectares, and 80% of the area is planted with pine (Pinus merkusii Jungh Et De Vriese). This paper reports the determination of the chemical composition of pine tree leaves. The method involves phytochemical screening including flavonoids, phenolic, saponin, steroids, terpenoid, and alkaloids and liquid chromatography mass spectrometry to determine the content of compounds present in this pine leaves extract. In addition, an evaluation of the antibacterial extract was also reported. It was found that variations in secondary metabolites were detected in each extract prepared from different parts of the plant, and their potential as an antibacterial agent was also large. The chemical composition was also confirmed from mass spectrum analysis. While the antibacterial activity shows important findings for future development.