Optimizing of lignin peroxidase production by the suspected novel strain of phanerochaete chrysosporium ITB isolate

Phanerochaete chrysosporium ITB isolate that suspected as the novel strain of P. chrysosporium, potential as a source of lignin peroxidase (LiP). Several conditions to optimize the production of LiP in submerge batch bioreactor, such as inoculum development, carbon source, temperature, agitation, pH, surfactant (Tween-80) and addition of activity enhancers (veratryl alcohol) were tested in this experiment. The spores of P. chrysosporium ITB isolate from optimum inoculum development inoculated in modified Tien Kirk medium to produce LiP. It was shown that the optimum cultivation time to produce optimum inoculum development was two weeks since the highest number of spores produced (3. 53. 10⁷ spores/mL) and the viability of the spores was still over 90%. Highest specific activity of LiP from P. chrysosporium ITB isolate was 77. 4 ± 13. 1 U/mg, achieved at optimized condition: The medium consist of sawdust 1%, ammonium sulfate 20 mM, tiamin-HCl 0, 01%, veratryl alcohol 300 ppm, Tween-80 0, 025%, basal medium and trace element components were the same as Kirk’s medium but in aqua demineralization, cultivated to 1. 10⁵ spore/mL, and were grown at 37 °C and 50 rpm for five days. The result depict that P. chrysosporium ITB isolate can produce high activity of lignin peroxidase in modified Tien Kirk medium.