Optimization of protein and tannin extraction in moringa oleifera leaf as antioxidant source

Moringa oleifera contains high nutritional and bioactive compounds that have the potential as a source of antioxidants. The main objective of this study was to obtain optimal conditions in the extraction process of Moringa leaf, maintain protein and antioxidant activity, and reduce tannin. It is carried out through the response surface method which consists of two factors. The first factor (X₁) was the extraction temperature (°C) which contains three levels, namely 70, 80, and 90°C. The second factor (X₂) was time (mins) consisting of three levels, namely 5, 10, and 15 mins. The parameters in this study were total protein, antioxidant activity, and total tannin. The results revealed that the optimum condition for Moringa leaf extraction is found to be temperature 80.54°C and a time of 12.19 mins. In that treatment, the total protein is 17.4594%, antioxidant activity is up to 10.2629 µg/mL, and tannin is 7.853% with the desirability of 0.792 or 79.2%.