Morphometric asymmetry of Barbodes binotatus (cyprinidae) collected from three different rivers in Java

Barbodes binotatus (Bleeker, 1859) is an endemic freshwater fish species that currently used for aquaculture. Good quality seed can be attained from cross-breed of parental stock from different geographical areas. This study aimed to distinguish morphological asymmetry of the species collected from three different rivers in Java: Sungai Tawang (East Java); Sungai Opak (Central Java); and Sungai Cisanggarung (West Java). Total 30 specimen were randomly sub-sampled from each river. Morphology, morphometry and meristic characters, based on 14 anatomical landmarks were measured and analyzed using Thin Plate Spline (TPSDIG), Symmetry-Asymmetry Geometric Analysis (SAGE) and Principal Component Analysis (PCA). Result of SAGE analysis showed that there were no significant asymmetries observed within, and between regions, respectively (p > 0.05). The result of PCA analysis however, showed qualitative differences in ratio between eye diameter (ED) versus head length (HL) (49%) and total length (TL) versus standard length (SL) (17%). Genetic (through DNA analysis) study need to be elaborated to see the differences more accurately.