Monolithic columns for the separation and analysis of proteins- A review

General protein preparation and analysis methods still have deficiencies regarding sensitivity and accuracy. Monolith columns are the best choice for the separation and analysis of proteins and its amino acids because they have many advantages of resolution, efficiency, and high sensitivity. Monolith columns can be used as stationary phase in chromatography due to easy preparation, high permeability, lower flow resistance and low back pressure. Monolith columns also can be used to clean up and for extraction from complex mixtures. This review summarizes the application of monolith columns in the separation and analysis of proteins as well as amino acids using chromatography.