Molecular characterization of litopenaeus vannamei molt inhibiting hormone (Mih) gene in seawater and freshwater aquaculture

The purpose of this research was to analyze the molecular characterization of Litopenaeus vannamei MIH gene that reared in seawater and freshwater aquaculture. Samples of L vannamei were taken from ponds at Gresik Regency and Situbondo Regency, East Java, Indonesia. Shrimp samples were taken to the Central Laboratory of Life Sciences, University of Brawijaya Malang for PCR testing. Molecular character of MIH from vanamei shrimp samples cultivated in seawater and freshwater was identified as MIH gene in vanamei shrimp with cover queries of 95-99%, identity values between 99-100 and E-value 0.0. Based on the results of electrophoresis, the MIH gene is found in basepair 212-237 .