Modification of monolithic stationary phase using human serum albumin as chiral separation

Dias Septiana; Stevin C. Angga; Suci Amalia; Elvina D. Iftitah; Akhmad Sabarudin

doi:10.1063/1.5062754

Modification of monolithic stationary phase using human serum albumin as chiral separation

Dias Septiana
, Stevin C. Angga
, Suci Amalia
, Elvina D. Iftitah
, Akhmad Sabarudin^*

^*Corresponding author for this work

Faculty of Mathematics and Natural Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

Enantiomers can have different pharmacological effects. Therefore, it is necessary to be able to separate pure enantiomers that have a beneficial biological activity. The purpose of this study was to modify a monolith column in stationary phase with Human Serum Albumin (HSA) for use in separating chiral compounds by HPLC. In this work, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) and poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly(GMA-co-TRIM)) columns were in-situ co-polymerized inside a silicosteel tubing. A total monomer ratio (% T) and crosslinking agent (% C) 40:25 and 28:12 were applied for the preparation of poly(GMA-co-EDMA) and poly(GMA-co-TRIM), respectively. The porogen used was 1-propanol, 1,4-butanediol, and water in a ratio of 7:4:1 (v/v) and AIBN as an initiator (1% of the total monomer). The column was polymerized at 60°C for 12 h, then immobilized with various concentrations of Human Serum Albumin (HSA). The monolithic poly(GMA-co-TRIM) column modified with HSA 1 mg/ml was successfully applied to separation of the enantiomers of citronellal using an acetonitrile and water (50:50, v/v) as the mobile phase at 0.05 ml/min. The permeability of this optimized monolithic column was 0.0018 Darcy.

Original language	English
Title of host publication	8th Annual Basic Science International Conference
Subtitle of host publication	Coverage of Basic Sciences toward the World's Sustainability Challenges
Editors	Corina Karim, Rodliyati Azrianingsih, Mauludi Ariesto Pamungkas, Yoga Dwi Jatmiko, Anna Safitri
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735417397
DOIs	https://doi.org/10.1063/1.5062754
Publication status	Published - 17 Oct 2018
Event	8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018 - Malang, East Java, Indonesia Duration: 6 Mar 2018 → 7 Mar 2018

Publication series

Name	AIP Conference Proceedings
Volume	2021
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018
Country/Territory	Indonesia
City	Malang, East Java
Period	6/03/18 → 7/03/18

Keywords

albumin
chiral separation
monolith column
stationary phase

Access to Document

10.1063/1.5062754

Cite this

Septiana, D., Angga, S. C., Amalia, S., Iftitah, E. D., & Sabarudin, A. (2018). Modification of monolithic stationary phase using human serum albumin as chiral separation. In C. Karim, R. Azrianingsih, M. A. Pamungkas, Y. D. Jatmiko, & A. Safitri (Eds.), 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges Article 050004 (AIP Conference Proceedings; Vol. 2021). American Institute of Physics Inc.. https://doi.org/10.1063/1.5062754

Septiana, Dias ; Angga, Stevin C. ; Amalia, Suci et al. / Modification of monolithic stationary phase using human serum albumin as chiral separation. 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges. editor / Corina Karim ; Rodliyati Azrianingsih ; Mauludi Ariesto Pamungkas ; Yoga Dwi Jatmiko ; Anna Safitri. American Institute of Physics Inc., 2018. (AIP Conference Proceedings).

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title = "Modification of monolithic stationary phase using human serum albumin as chiral separation",

abstract = "Enantiomers can have different pharmacological effects. Therefore, it is necessary to be able to separate pure enantiomers that have a beneficial biological activity. The purpose of this study was to modify a monolith column in stationary phase with Human Serum Albumin (HSA) for use in separating chiral compounds by HPLC. In this work, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) and poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly(GMA-co-TRIM)) columns were in-situ co-polymerized inside a silicosteel tubing. A total monomer ratio (\% T) and crosslinking agent (\% C) 40:25 and 28:12 were applied for the preparation of poly(GMA-co-EDMA) and poly(GMA-co-TRIM), respectively. The porogen used was 1-propanol, 1,4-butanediol, and water in a ratio of 7:4:1 (v/v) and AIBN as an initiator (1\% of the total monomer). The column was polymerized at 60°C for 12 h, then immobilized with various concentrations of Human Serum Albumin (HSA). The monolithic poly(GMA-co-TRIM) column modified with HSA 1 mg/ml was successfully applied to separation of the enantiomers of citronellal using an acetonitrile and water (50:50, v/v) as the mobile phase at 0.05 ml/min. The permeability of this optimized monolithic column was 0.0018 Darcy.",

keywords = "albumin, chiral separation, monolith column, stationary phase",

author = "Dias Septiana and Angga, \{Stevin C.\} and Suci Amalia and Iftitah, \{Elvina D.\} and Akhmad Sabarudin",

note = "Publisher Copyright: {\textcopyright} 2018 Author(s).; 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018 ; Conference date: 06-03-2018 Through 07-03-2018",

year = "2018",

month = oct,

day = "17",

doi = "10.1063/1.5062754",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Corina Karim and Rodliyati Azrianingsih and Pamungkas, \{Mauludi Ariesto\} and Jatmiko, \{Yoga Dwi\} and Anna Safitri",

booktitle = "8th Annual Basic Science International Conference",

}

Septiana, D, Angga, SC, Amalia, S, Iftitah, ED & Sabarudin, A 2018, Modification of monolithic stationary phase using human serum albumin as chiral separation. in C Karim, R Azrianingsih, MA Pamungkas, YD Jatmiko & A Safitri (eds), 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges., 050004, AIP Conference Proceedings, vol. 2021, American Institute of Physics Inc., 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018, Malang, East Java, Indonesia, 6/03/18. https://doi.org/10.1063/1.5062754

Modification of monolithic stationary phase using human serum albumin as chiral separation. / Septiana, Dias; Angga, Stevin C.; Amalia, Suci et al.
8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges. ed. / Corina Karim; Rodliyati Azrianingsih; Mauludi Ariesto Pamungkas; Yoga Dwi Jatmiko; Anna Safitri. American Institute of Physics Inc., 2018. 050004 (AIP Conference Proceedings; Vol. 2021).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Modification of monolithic stationary phase using human serum albumin as chiral separation

AU - Septiana, Dias

AU - Angga, Stevin C.

AU - Amalia, Suci

AU - Iftitah, Elvina D.

AU - Sabarudin, Akhmad

PY - 2018/10/17

Y1 - 2018/10/17

N2 - Enantiomers can have different pharmacological effects. Therefore, it is necessary to be able to separate pure enantiomers that have a beneficial biological activity. The purpose of this study was to modify a monolith column in stationary phase with Human Serum Albumin (HSA) for use in separating chiral compounds by HPLC. In this work, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) and poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly(GMA-co-TRIM)) columns were in-situ co-polymerized inside a silicosteel tubing. A total monomer ratio (% T) and crosslinking agent (% C) 40:25 and 28:12 were applied for the preparation of poly(GMA-co-EDMA) and poly(GMA-co-TRIM), respectively. The porogen used was 1-propanol, 1,4-butanediol, and water in a ratio of 7:4:1 (v/v) and AIBN as an initiator (1% of the total monomer). The column was polymerized at 60°C for 12 h, then immobilized with various concentrations of Human Serum Albumin (HSA). The monolithic poly(GMA-co-TRIM) column modified with HSA 1 mg/ml was successfully applied to separation of the enantiomers of citronellal using an acetonitrile and water (50:50, v/v) as the mobile phase at 0.05 ml/min. The permeability of this optimized monolithic column was 0.0018 Darcy.

AB - Enantiomers can have different pharmacological effects. Therefore, it is necessary to be able to separate pure enantiomers that have a beneficial biological activity. The purpose of this study was to modify a monolith column in stationary phase with Human Serum Albumin (HSA) for use in separating chiral compounds by HPLC. In this work, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) and poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly(GMA-co-TRIM)) columns were in-situ co-polymerized inside a silicosteel tubing. A total monomer ratio (% T) and crosslinking agent (% C) 40:25 and 28:12 were applied for the preparation of poly(GMA-co-EDMA) and poly(GMA-co-TRIM), respectively. The porogen used was 1-propanol, 1,4-butanediol, and water in a ratio of 7:4:1 (v/v) and AIBN as an initiator (1% of the total monomer). The column was polymerized at 60°C for 12 h, then immobilized with various concentrations of Human Serum Albumin (HSA). The monolithic poly(GMA-co-TRIM) column modified with HSA 1 mg/ml was successfully applied to separation of the enantiomers of citronellal using an acetonitrile and water (50:50, v/v) as the mobile phase at 0.05 ml/min. The permeability of this optimized monolithic column was 0.0018 Darcy.

KW - albumin

KW - chiral separation

KW - monolith column

KW - stationary phase

UR - https://www.scopus.com/pages/publications/85056174093

U2 - 10.1063/1.5062754

DO - 10.1063/1.5062754

M3 - Conference contribution

AN - SCOPUS:85056174093

T3 - AIP Conference Proceedings

BT - 8th Annual Basic Science International Conference

A2 - Karim, Corina

A2 - Azrianingsih, Rodliyati

A2 - Pamungkas, Mauludi Ariesto

A2 - Jatmiko, Yoga Dwi

A2 - Safitri, Anna

PB - American Institute of Physics Inc.

T2 - 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018

Y2 - 6 March 2018 through 7 March 2018

ER -

Septiana D, Angga SC, Amalia S, Iftitah ED , Sabarudin A. Modification of monolithic stationary phase using human serum albumin as chiral separation. In Karim C, Azrianingsih R, Pamungkas MA, Jatmiko YD, Safitri A, editors, 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challenges. American Institute of Physics Inc. 2018. 050004. (AIP Conference Proceedings). doi: 10.1063/1.5062754

Modification of monolithic stationary phase using human serum albumin as chiral separation

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