Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression

Agustina Tri Endharti; Eni Sulastri; Rabiah Umanailo; Yunialce; Tatit Nurseta; Kusworini Handono

doi:10.7324/JAPS.2018.8702

Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression

Agustina Tri Endharti^*
, Eni Sulastri
, Rabiah Umanailo
, Yunialce
, Tatit Nurseta
, Kusworini Handono

^*Corresponding author for this work

Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Mango mistletoe (Dendrophthoe pentandra L. Miq) (MMDP) leaf extract which contained quercetin inhibits cancer cells. MMDP increasing effectively of 5-Fluorouracil (5-FU) as a chemotherapy agent. This study aimed to investigate the synergistic effects of MMDP combined with 5-FU to determine the percentage of apoptosis, p21, and survivin in HeLa cells. Flow cytometry analysis revealed that it increases apoptosis HeLa cell through increasing p21 and also decreased survivin percentage. The combination of 5-FU 5 μg/ml and MMDP 50 μg/ml significantly increased the percentage of p21 and apoptosis (P < 0.001; P < 0.001) and decreased survivin (P < 0.05) compared with 5-FU only group. This study indicates that the combined 5-FU and MMDP has a synergistic effect to enhance apoptosis and p21 expressions by decreasing of survivin in HeLa cells.

Original language	English
Pages (from-to)	10-15
Number of pages	6
Journal	Journal of Applied Pharmaceutical Science
Volume	8
Issue number	7
DOIs	https://doi.org/10.7324/JAPS.2018.8702
Publication status	Published - 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

5-FU
Apoptosis
Mango mistletoe (Dendrophthoe pentandra)
P21
Survivin

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Endharti, A. T., Sulastri, E., Umanailo, R., Yunialce, Nurseta, T., & Handono, K. (2018). Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression. Journal of Applied Pharmaceutical Science, 8(7), 10-15. https://doi.org/10.7324/JAPS.2018.8702

@article{e8e44dede2a3430b8df3beb386f333d8,

title = "Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression",

abstract = "Mango mistletoe (Dendrophthoe pentandra L. Miq) (MMDP) leaf extract which contained quercetin inhibits cancer cells. MMDP increasing effectively of 5-Fluorouracil (5-FU) as a chemotherapy agent. This study aimed to investigate the synergistic effects of MMDP combined with 5-FU to determine the percentage of apoptosis, p21, and survivin in HeLa cells. Flow cytometry analysis revealed that it increases apoptosis HeLa cell through increasing p21 and also decreased survivin percentage. The combination of 5-FU 5 μg/ml and MMDP 50 μg/ml significantly increased the percentage of p21 and apoptosis (P < 0.001; P < 0.001) and decreased survivin (P < 0.05) compared with 5-FU only group. This study indicates that the combined 5-FU and MMDP has a synergistic effect to enhance apoptosis and p21 expressions by decreasing of survivin in HeLa cells.",

keywords = "5-FU, Apoptosis, Mango mistletoe (Dendrophthoe pentandra), P21, Survivin",

author = "Endharti, \{Agustina Tri\} and Eni Sulastri and Rabiah Umanailo and Yunialce and Tatit Nurseta and Kusworini Handono",

note = "Publisher Copyright: {\textcopyright} 2018 Agustina Tri Endharti et al.",

year = "2018",

doi = "10.7324/JAPS.2018.8702",

language = "English",

volume = "8",

pages = "10--15",

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Endharti, AT, Sulastri, E, Umanailo, R, Yunialce, , Nurseta, T & Handono, K 2018, 'Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression', Journal of Applied Pharmaceutical Science, vol. 8, no. 7, pp. 10-15. https://doi.org/10.7324/JAPS.2018.8702

Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression. / Endharti, Agustina Tri; Sulastri, Eni; Umanailo, Rabiah et al.
In: Journal of Applied Pharmaceutical Science, Vol. 8, No. 7, 2018, p. 10-15.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression

AU - Endharti, Agustina Tri

AU - Sulastri, Eni

AU - Umanailo, Rabiah

AU - Yunialce,

AU - Nurseta, Tatit

AU - Handono, Kusworini

PY - 2018

Y1 - 2018

N2 - Mango mistletoe (Dendrophthoe pentandra L. Miq) (MMDP) leaf extract which contained quercetin inhibits cancer cells. MMDP increasing effectively of 5-Fluorouracil (5-FU) as a chemotherapy agent. This study aimed to investigate the synergistic effects of MMDP combined with 5-FU to determine the percentage of apoptosis, p21, and survivin in HeLa cells. Flow cytometry analysis revealed that it increases apoptosis HeLa cell through increasing p21 and also decreased survivin percentage. The combination of 5-FU 5 μg/ml and MMDP 50 μg/ml significantly increased the percentage of p21 and apoptosis (P < 0.001; P < 0.001) and decreased survivin (P < 0.05) compared with 5-FU only group. This study indicates that the combined 5-FU and MMDP has a synergistic effect to enhance apoptosis and p21 expressions by decreasing of survivin in HeLa cells.

AB - Mango mistletoe (Dendrophthoe pentandra L. Miq) (MMDP) leaf extract which contained quercetin inhibits cancer cells. MMDP increasing effectively of 5-Fluorouracil (5-FU) as a chemotherapy agent. This study aimed to investigate the synergistic effects of MMDP combined with 5-FU to determine the percentage of apoptosis, p21, and survivin in HeLa cells. Flow cytometry analysis revealed that it increases apoptosis HeLa cell through increasing p21 and also decreased survivin percentage. The combination of 5-FU 5 μg/ml and MMDP 50 μg/ml significantly increased the percentage of p21 and apoptosis (P < 0.001; P < 0.001) and decreased survivin (P < 0.05) compared with 5-FU only group. This study indicates that the combined 5-FU and MMDP has a synergistic effect to enhance apoptosis and p21 expressions by decreasing of survivin in HeLa cells.

KW - 5-FU

KW - Apoptosis

KW - Mango mistletoe (Dendrophthoe pentandra)

KW - P21

KW - Survivin

UR - https://www.scopus.com/pages/publications/85055455837

U2 - 10.7324/JAPS.2018.8702

DO - 10.7324/JAPS.2018.8702

M3 - Article

AN - SCOPUS:85055455837

SN - 2231-3354

VL - 8

SP - 10

EP - 15

JO - Journal of Applied Pharmaceutical Science

JF - Journal of Applied Pharmaceutical Science

IS - 7

ER -

Endharti AT, Sulastri E, Umanailo R, Yunialce , Nurseta T , Handono K. Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression. Journal of Applied Pharmaceutical Science. 2018;8(7):10-15. doi: 10.7324/JAPS.2018.8702

Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression

Abstract

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Keywords

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