Male calf proportion of artificial insemination results by using sexed sperm with double dose on Ongole crosbred cows

The technology of artificial insemination is very popular to increase the population of cattle in Indonesia. This technology could be more valuable with sexing sperms in order to get the offspring with expected sex. The aim of the research is to know the proportion of male calves that was born from crossbred Ongolecows which were inseminated using double dosesemen sexed for Y sperm. The materials were 43 calves from Artificial Insemination Center Singosari. Twenty four calves was born from cows inseminated with non-sexed semen and nineteen calves with sexed semen. The percentage of sperm carrying X and Y chromosomes was performed by measuring the size of the sperm's head by using a microscope. The result showed that the proportion of Y sperm in sexed semen was 80.79 %, while the X sperms was 19.21%. Furthermore, the percentages of the male calves that born from double dose sexed Y sperm was 78.95 %, while from the non sexing-semen was 21.05%. It can be conluded that the artificial insemination using double dose sexed semen can result the male calves proportionate with the Y sperm proportion in the sexed semen.