TY - GEN
T1 - Low dose nicotine lower MDA level and does not affect wall to lumen ratio in surgically menopausal rats
AU - Ernanda, Shelby Amrus
AU - Sananta, Panji
AU - Jonatan, Andrew
AU - Kartikaningtyas, Ayu Novita
AU - Parhusip, Yanti Marito
AU - Baskarani, Ni Putu Frida
AU - Andhira, Nadya Rasty
AU - Maulidya, Elli
AU - Amelia, Yesi
AU - Vico, Muhammad
AU - Hudayana, Nur
AU - Juwono, Muthiah Adira
AU - Rosyida, Iis Novianti
AU - Putra, Ramendra Dirgantara
AU - Aziz, Azmi
AU - Winarsih, Sri
AU - Rizal, Ardian
N1 - Publisher Copyright:
© 2020 American Institute of Physics Inc.. All rights reserved.
PY - 2020/4/21
Y1 - 2020/4/21
N2 - Menopause is a condition where a woman lost protection from estrogen, causing susceptibility to arterial stenosis (atherosclerosis) induced by increased free radicals' activity and inflammation. It was reported that nicotine can reduce free radicals' activity and act as an anti-inflammatory agent. This study means to explore the possibility of nicotine usage to prevent atherosclerosis and to reduce free radical activity. We did experimental randomized post-test only controlled group research in 18-20 weeks old female Rattus norvegicus. We examined tail arterial histomorphometry, Wall to Lumen Ratio (WTLR), and Malondialdehyde (MDA) level of abdominal aorta as markers of free radical activity. The first group was normal rats (Sham). The second group represents the ovariectomy group (ovariectomy-only/Ovx). The third to the fifth group were ovariectomized and given nicotine in a dose of 0.25 mg/kg body weight (P1), 0.5 mg/kg (P2), and 0.75 mg/kg (P3). Group P1-P3 was given daily nicotine by oral route for 4 weeks, started from 3 weeks post-ovariectomy. MDA levels of P3 were significantly lower than Ovx group (p = 0.036, Tukey HSD). WTLR measurement found no significant differences across all groups. Finally, we conclude that nicotine has antioxidant properties and does not affect tail artery WTLR.
AB - Menopause is a condition where a woman lost protection from estrogen, causing susceptibility to arterial stenosis (atherosclerosis) induced by increased free radicals' activity and inflammation. It was reported that nicotine can reduce free radicals' activity and act as an anti-inflammatory agent. This study means to explore the possibility of nicotine usage to prevent atherosclerosis and to reduce free radical activity. We did experimental randomized post-test only controlled group research in 18-20 weeks old female Rattus norvegicus. We examined tail arterial histomorphometry, Wall to Lumen Ratio (WTLR), and Malondialdehyde (MDA) level of abdominal aorta as markers of free radical activity. The first group was normal rats (Sham). The second group represents the ovariectomy group (ovariectomy-only/Ovx). The third to the fifth group were ovariectomized and given nicotine in a dose of 0.25 mg/kg body weight (P1), 0.5 mg/kg (P2), and 0.75 mg/kg (P3). Group P1-P3 was given daily nicotine by oral route for 4 weeks, started from 3 weeks post-ovariectomy. MDA levels of P3 were significantly lower than Ovx group (p = 0.036, Tukey HSD). WTLR measurement found no significant differences across all groups. Finally, we conclude that nicotine has antioxidant properties and does not affect tail artery WTLR.
UR - https://www.scopus.com/pages/publications/85092575241
U2 - 10.1063/5.0003164
DO - 10.1063/5.0003164
M3 - Conference contribution
AN - SCOPUS:85092575241
T3 - AIP Conference Proceedings
BT - International Conference on Life Science and Technology, ICoLiST 2019
A2 - Taufiq, Ahmad
A2 - Susanto, Hendra
A2 - Nur, Hadi
A2 - Aziz, Muhammad
A2 - Chang, Chuang-Rung
A2 - Lee, Hyohyemi
A2 - Diantoro, Markus
A2 - Mufti, Nandang
A2 - Malek, Nik Ahmad Nizam Nik
A2 - Wang, I. Ching
A2 - Iskandar, Djoko Tjahnono
A2 - Elbers, Gereon
A2 - Sunaryono, Sunaryono
A2 - Zubaidah, Siti
A2 - Sumari, Sumari
A2 - Aulanni'am, null
A2 - Nandiyanto, Asep Bayu
A2 - Wibowo, Indra
A2 - Handaya, Adeodatus Yuda
PB - American Institute of Physics Inc.
T2 - 2nd International Conference on Life Science and Technology, ICoLiST 2019
Y2 - 12 September 2019 through 13 September 2019
ER -