Isolation and identification structure antioxidant active compounds of ethyl acetate fraction hypokotil Bruguiera gymnorhiza (L) Lamk

Bruguiera gymnorhiza (L) Lamk is one of mangrove plants that commonly used as traditional food and medicine. As traditional medicine, it needs information on contained compound in the hypocotyls of B. gymnorhiza. In general, compound insulation only applied to the flowers, barks and leaves of B. gymnorhiza, while isolation from hypocotyls was not much practiced. The existence of antioxidant compounds in hypocotyls of B. gymnorhiza was essential information for the development of functional food and pharmaceuticals. This study aimed to isolate and identify the structure of the active compounds of the most active antioxidant in ethyl acetate fraction hypocotyls of B. gymnorhiza. Methods for isolation and identification of active antioxidant compounds are: maceration, evaporation, partitioning, and the structure determination of the compound. The results showed that the fraction of F4.80 to F4.120 produces a single spot stains and high antioxidant activity. Structure identification of the active antioxidant compounds was performed using ¹³C-NMR spectrum and ₁H-NMR 2D. Based on the results of the reading on 135º DEPT, ¹H-¹H-COSY, HMQC, and HMBC, the compound target has 29 carbon atoms, 50 hydrogen atoms and two oxygen atoms with molecular formula C₂₉H₅₀O₂. This compound is a derivative phenolic compound with molecular structure resembles the structure of the compound of α-tocopherol.