Abstract
The aim of this study is to isolate, characterize and identify the lipase-degrading bacteria from fish canning wastewater. From this study, seven isolates of lipase-degrading bacteria were obtained. The high lipase-degrading activity was showed by isolate AG-2 and AD-1 with activity of 12.22 U/mL and 11.11 U/mL, respectively. Both isolates then characterized for the optimum temperature and pH. The result showed that the optimum temperature for growth of isolate AG-2 was 27°C, whereas isolate AD-1 was 37°C. Both of the isolates have the same pH for optimum growth, pH 8. Because the optimum temperature of isolate AD-1 has the same condition with temperature of sample, isolate AD-1 is chosen as alternative for biological wastewater treatment. Molecular identification of isolate AD-1 using 16S rRNA sequencing showed that it has 99% similarity with Bacillus cereus SBD1-8.
| Original language | English |
|---|---|
| Pages (from-to) | 441-446 |
| Number of pages | 6 |
| Journal | International Journal on Advanced Science, Engineering and Information Technology |
| Volume | 6 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2016 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 6 Clean Water and Sanitation
Keywords
- 16S rRNA
- Characterization
- Fish canning wastewater
- Identification
- Isolation
- Lipid-degrading bacteria
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