In vitro study on the inhibitory effect of ketamine to LPS-induced apoptosis in HUVEC culture

Aswoco Andyk Asmoro; Rudy Vitraludyono; Redhy Sindharta; Muhammad Aris Widodo; Siti Chasnak Saleh; Edi Widjayanto

doi:10.1016/S2222-1808(15)60966-5

In vitro study on the inhibitory effect of ketamine to LPS-induced apoptosis in HUVEC culture

Aswoco Andyk Asmoro^*
, Rudy Vitraludyono
, Redhy Sindharta
, Muhammad Aris Widodo
, Siti Chasnak Saleh
, Edi Widjayanto

^*Corresponding author for this work

Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Objective: To determine the effect of lipopolysaccharide (LPS) exposure and treatment with ketamine to the levels of nitric oxide, intracellular calcium and caspase-3 of human umbilical vein endothelial cell culture. Methods: This research used the human umbilical vein endothelial cell culture with LPS induction. ELISA was conducted to measure the levels of apoptosis markers such as nitric oxide, intracellular calcium and caspase-3. Results: The results showed significant elevation of all parameters after LPS induction. Ketamine addition resulted in various effects. Measurement of intracellular calcium and caspase-3 showed similar result, in which the inhibitory effect of ketamine was more effective when added at the same time as LPS. Vice versa, in nitric oxide measurement, the highest inhibitory effect of ketamine was obtained when added 6 h after LPS induction. Conclusions: At least in part, ketamine was suggested to have inhibitory effect in apoptosis mechanism.

Original language	English
Pages (from-to)	968-970
Number of pages	3
Journal	Asian Pacific Journal of Tropical Disease
Volume	5
Issue number	12
DOIs	https://doi.org/10.1016/S2222-1808(15)60966-5
Publication status	Published - 1 Dec 2015

Keywords

Apoptosis
HUVEC
Ketamine
LPS
Sepsis

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10.1016/S2222-1808(15)60966-5

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title = "In vitro study on the inhibitory effect of ketamine to LPS-induced apoptosis in HUVEC culture",

abstract = "Objective: To determine the effect of lipopolysaccharide (LPS) exposure and treatment with ketamine to the levels of nitric oxide, intracellular calcium and caspase-3 of human umbilical vein endothelial cell culture. Methods: This research used the human umbilical vein endothelial cell culture with LPS induction. ELISA was conducted to measure the levels of apoptosis markers such as nitric oxide, intracellular calcium and caspase-3. Results: The results showed significant elevation of all parameters after LPS induction. Ketamine addition resulted in various effects. Measurement of intracellular calcium and caspase-3 showed similar result, in which the inhibitory effect of ketamine was more effective when added at the same time as LPS. Vice versa, in nitric oxide measurement, the highest inhibitory effect of ketamine was obtained when added 6 h after LPS induction. Conclusions: At least in part, ketamine was suggested to have inhibitory effect in apoptosis mechanism.",

keywords = "Apoptosis, HUVEC, Ketamine, LPS, Sepsis",

author = "Asmoro, \{Aswoco Andyk\} and Rudy Vitraludyono and Redhy Sindharta and Widodo, \{Muhammad Aris\} and Saleh, \{Siti Chasnak\} and Edi Widjayanto",

note = "Publisher Copyright: {\textcopyright} 2015 Asian Pacific Tropical Medicine Press.",

year = "2015",

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day = "1",

doi = "10.1016/S2222-1808(15)60966-5",

language = "English",

volume = "5",

pages = "968--970",

journal = "Asian Pacific Journal of Tropical Disease",

issn = "2222-1808",

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TY - JOUR

T1 - In vitro study on the inhibitory effect of ketamine to LPS-induced apoptosis in HUVEC culture

AU - Asmoro, Aswoco Andyk

AU - Vitraludyono, Rudy

AU - Sindharta, Redhy

AU - Widodo, Muhammad Aris

AU - Saleh, Siti Chasnak

AU - Widjayanto, Edi

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Objective: To determine the effect of lipopolysaccharide (LPS) exposure and treatment with ketamine to the levels of nitric oxide, intracellular calcium and caspase-3 of human umbilical vein endothelial cell culture. Methods: This research used the human umbilical vein endothelial cell culture with LPS induction. ELISA was conducted to measure the levels of apoptosis markers such as nitric oxide, intracellular calcium and caspase-3. Results: The results showed significant elevation of all parameters after LPS induction. Ketamine addition resulted in various effects. Measurement of intracellular calcium and caspase-3 showed similar result, in which the inhibitory effect of ketamine was more effective when added at the same time as LPS. Vice versa, in nitric oxide measurement, the highest inhibitory effect of ketamine was obtained when added 6 h after LPS induction. Conclusions: At least in part, ketamine was suggested to have inhibitory effect in apoptosis mechanism.

AB - Objective: To determine the effect of lipopolysaccharide (LPS) exposure and treatment with ketamine to the levels of nitric oxide, intracellular calcium and caspase-3 of human umbilical vein endothelial cell culture. Methods: This research used the human umbilical vein endothelial cell culture with LPS induction. ELISA was conducted to measure the levels of apoptosis markers such as nitric oxide, intracellular calcium and caspase-3. Results: The results showed significant elevation of all parameters after LPS induction. Ketamine addition resulted in various effects. Measurement of intracellular calcium and caspase-3 showed similar result, in which the inhibitory effect of ketamine was more effective when added at the same time as LPS. Vice versa, in nitric oxide measurement, the highest inhibitory effect of ketamine was obtained when added 6 h after LPS induction. Conclusions: At least in part, ketamine was suggested to have inhibitory effect in apoptosis mechanism.

KW - Apoptosis

KW - HUVEC

KW - Ketamine

KW - LPS

KW - Sepsis

UR - https://www.scopus.com/pages/publications/84960382629

U2 - 10.1016/S2222-1808(15)60966-5

DO - 10.1016/S2222-1808(15)60966-5

M3 - Article

AN - SCOPUS:84960382629

SN - 2222-1808

VL - 5

SP - 968

EP - 970

JO - Asian Pacific Journal of Tropical Disease

JF - Asian Pacific Journal of Tropical Disease

IS - 12

ER -

In vitro study on the inhibitory effect of ketamine to LPS-induced apoptosis in HUVEC culture

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Keywords

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